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    alkbh7-antibody-ab204568.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair DNA Damage Response Other
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Anti-ALKBH7 antibody (ab204568)

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Western blot - Anti-ALKBH7 antibody (ab204568)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALKBH7 antibody (ab204568)

Key features and details

  • Rabbit polyclonal to ALKBH7
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Protein
Product image
Recombinant Human ALKBH7 protein (ab164545)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-ALKBH7 antibody
    See all ALKBH7 primary antibodies
  • Description

    Rabbit polyclonal to ALKBH7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment corresponding to Human ALKBH7 aa 21-92.
    Sequence:

    SGPSVLSRLQDAAVVRPGFLSTAEEETLSRELEPELRRRRYEYDHWDAAI HGFRETEKSRWSEASRAILQRV


    Database link: Q9BT30
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human kidney tissue; LY403154 cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human ALKBH7 protein (ab164545)
  • Related Products

    • Recombinant Human ALKBH7 protein (ab164545)

Applications

Our Abpromise guarantee covers the use of ab204568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 25 kDa.

Target

  • Function

    Probable dioxygenase that requires molecular oxygen, alpha-ketoglutarate and iron.
  • Tissue specificity

    Widely expressed, with highest expression in pancreas, followed by spleen, prostate, ovary and placenta.
  • Sequence similarities

    Belongs to the alkB family.
  • Cellular localization

    Cytoplasm. Nucleus. Secreted. Has a predicted N-terminal signal sequence, indicating it may be secreted. Detected in cytoplasm and nucleus when expressed as fusion protein with an N-terminal tag.
  • Target information above from: UniProt accession Q9BT30 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 84266 Human
    • Omim: 613305 Human
    • SwissProt: Q2M2S8 Cow
    • SwissProt: Q9BT30 Human
    • Unigene: 111099 Human
    • Alternative names

      • ABH7 antibody
      • ALKB7_HUMAN antibody
      • ALKBH7 antibody
      • Alkylated DNA repair protein alkB homolog 7 antibody
      • MGC10974 antibody
      • Probable alpha-ketoglutarate-dependent dioxygenase ABH7 antibody
      • SPATA11 antibody
      • Spermatogenesis cell proliferation-related protein antibody
      • Spermatogenesis-associated protein 11 antibody
      • UNQ6002 antibody
      • UNQ6002/PRO34564 antibody
      see all

    Images

    • Western blot - Anti-ALKBH7 antibody (ab204568)
      Western blot - Anti-ALKBH7 antibody (ab204568)
      All lanes : Anti-ALKBH7 antibody (ab204568) at 1/100 dilution

      Lane 1 : Negative Control
      Lane 2 : overexpression cell lysate

      Predicted band size: 25 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALKBH7 antibody (ab204568)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALKBH7 antibody (ab204568)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ALKBH7 with ab204568 at 1/50 dilution.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab204568? Please let us know so that we can cite the reference in this datasheet.

    ab204568 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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