Key features and details
- Rabbit polyclonal to ALKBH8
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ALKBH8 antibody
See all ALKBH8 primary antibodies
DescriptionRabbit polyclonal to ALKBH8
Specificityab113512 is also predicted to react with isoform 4 of Human ALKBH8 (76kDa).
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment, corresponding to N terminal amino acids 2-238 of Human ALKBH8 (BC015183).
- Human fetal spleen and A549 cell lysates; Human fetal spleen tissue.
Storage instructionsAvoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab113512 is purified by a protein affinity column.
Our Abpromise guarantee covers the use of ab113512 in the following tested applications.
|WB||1/500 - 1/1000. Predicted molecular weight: 75 kDa.|
|IHC-P||1/100 - 1/500.|
FunctionCatalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis.
Tissue specificityWidely expressed, with highest expression in spleen, followed by pancreas and lung.
Sequence similaritiesBelongs to the alkB family.
Contains 1 Fe2OG dioxygenase domain.
Contains 1 RRM (RNA recognition motif) domain.
Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic.
- Information by UniProt
- 4930562C03Rik antibody
- 8030431D03Rik antibody
- 9430088N01Rik antibody
All lanes : Anti-ALKBH8 antibody (ab113512) at 1/500 dilution
Lane 1 : Human fetal spleen lysate
Lane 2 : A549 cell lysate
Predicted band size: 75 kDa
ab113512 at 1/100 dilution staining ALKBH8 in Formalin-fixed, Paraffin-embedded Human fetal spleen tissue by Immunohistochemistry.
Note cytoplasmic and nuclear staining.
ab113512 has been referenced in 1 publication.
- Delaunay S et al. Elp3 links tRNA modification to IRES-dependent translation of LEF1 to sustain metastasis in breast cancer. J Exp Med 213:2503-2523 (2016). PubMed: 27811057