Product nameAlkyne Phosphoramidite, 5'-terminal, for the synthesis of oligonucleotides
DescriptionPhosphoramidite for the synthesis of oligonucleotides with 5'-terminal alkyne for Click Chemistry.
Phosphoramidite for the synthesis of oligonucleotides with 5'-terminal alkyne for Click Chemistry. Easier to handle and dispense, and more stable compared to 5’-hexynyl phosphoramidite, 5'-butynyl-CEP, and other 5'-terminal alkyne phosphoramidites. Use acetonitrile as diluent. A 5 minute coupling time is recommended using standard nucleobase conditions. 5'-deprotection not needed due to lack of 5'-terminal DMT group. Oligonucleotides can be deblocked under standard conditions and purified by PAGE or HPLC ion-exhange chromatography.
Storage instructionsShipped at Room Temperature. Store at -20°C. Store under desiccating conditions.
Acetonitrile (good solubility)
Dichloromethane (good solubility)
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
This product has been referenced in:
- Taskova M et al. Tandem Oligonucleotide Probe Annealing and Elongation To Discriminate Viral Sequence. Anal Chem 89:4363-4366 (2017). Read more (PubMed: 28382823) »
- Aparin IO et al. 1-Phenylethynylpyrene (PEPy) as a novel blue-emitting dye for qPCR assay. Analyst 141:1331-8 (2016). Read more (PubMed: 26738806) »
- Astakhova IK et al. Branched DNA nanostructures efficiently stabilised and monitored by novel pyrene-perylene 2'-a-L-amino-LNA FRET pairs. Chem Commun (Camb) 49:511-3 (2013). Read more (PubMed: 23201901) »