Alkyne Phosphoramidite, 5'-terminal, for the synthesis of oligonucleotides (ab235034)

Overview

  • Product name

    Alkyne Phosphoramidite, 5'-terminal, for the synthesis of oligonucleotides
  • Description

    Phosphoramidite for the synthesis of oligonucleotides with 5'-terminal alkyne for Click Chemistry.
  • Biological description

     


    Phosphoramidite for the synthesis of oligonucleotides with 5'-terminal alkyne for Click Chemistry. Easier to handle and dispense, and more stable compared to 5’-hexynyl phosphoramidite, 5'-butynyl-CEP, and other 5'-terminal alkyne phosphoramidites. Use acetonitrile as diluent. A 5 minute coupling time is recommended using standard nucleobase conditions. 5'-deprotection not needed due to lack of 5'-terminal DMT group. Oligonucleotides can be deblocked under standard conditions and purified by PAGE or HPLC ion-exhange chromatography.

  • Purity

    > 95%
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    trans-4-(5-Hexynoylamino)cyclohexyloxy-N,N-diisopropylamino-2-cyanoethyloxyphosphine 

  • Molecular weight

    409.50
  • Molecular formula

    C21H36N3O3P
  • Storage instructions

    Shipped at Room Temperature. Store at -20°C. Store under desiccating conditions.
  • Solubility overview

    Acetonitrile (good solubility)

    Dichloromethane (good solubility)

  • Handling

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

References

This product has been referenced in:

  • Taskova M  et al. Tandem Oligonucleotide Probe Annealing and Elongation To Discriminate Viral Sequence. Anal Chem 89:4363-4366 (2017). Read more (PubMed: 28382823) »
  • Aparin IO  et al. 1-Phenylethynylpyrene (PEPy) as a novel blue-emitting dye for qPCR assay. Analyst 141:1331-8 (2016). Read more (PubMed: 26738806) »
  • Astakhova IK  et al. Branched DNA nanostructures efficiently stabilised and monitored by novel pyrene-perylene 2'-a-L-amino-LNA FRET pairs. Chem Commun (Camb) 49:511-3 (2013). Read more (PubMed: 23201901) »

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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