Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 50% Glycerol, PBS, pH 7.5
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab53956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 20831795|
|IP||Use at an assay dependent concentration.|
|RIA||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
RelevanceAllatostatins (ASTs) are best known as neuropeptide Inhibitors of Juvenile Hormone Synthesis. They are a family of peptides first isolated from the cockroach Diploptera punctata and now known to be widely distributed among insect orders. In cockroaches, ASTs have a number of different functions. Their most widely documented role is the inhibition of juvenile hormone production by endocrine glands called the corpora allata (CA). ASTs also inhibit contractions of visceral muscle and most recently, they have been implicated as modulators of digestive enzyme production by the midgut. The allatostatins are specific to insects and thus may provide an opportunity to develop novel means of insect pest control.
- Allatostatin antibody
- AST1 antibody
Immunohistochemical analysis of Birgus latro (Coconut crab) brain tissue, staining Allatostatin 1 (green) with ab53956.
The brains were dissected in phosphate buffered saline (0.1 M PBS, pH 7.4) then fixed in 4% PFA in 0.1 PBS, pH 7.4 at 4°C before sectioning at room temperature. The sections were incubated for 90 minutes in PBS-TX (1% BS|A, 0.3% Triton X-100, 0.05% sodium azide, pH 7.4) followed by overnight incubation with primary antibody (1/1000 in PBS-TX) at room temperature. An AlexaFluor®488-conjugated secondary antibody was used to detect staining.
This product has been referenced in:
- Krieger J et al. Brain architecture of the largest living land arthropod, the Giant Robber Crab Birgus latro (Crustacea, Anomura, Coenobitidae): evidence for a prominent central olfactory pathway? Front Zool 7:25 (2010). ICC/IF ; Other species . Read more (PubMed: 20831795) »