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Anti-Allergin 1 antibody (ab229951)

  • Datasheet
  • SDS
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Abpromise

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Western blot - Anti-Allergin 1 antibody (ab229951)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Allergin 1 antibody (ab229951)
  • Immunocytochemistry/ Immunofluorescence - Anti-Allergin 1 antibody (ab229951)

Key features and details

  • Rabbit polyclonal to Allergin 1
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Allergin 1 antibody
  • Description

    Rabbit polyclonal to Allergin 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human Allergin 1 aa 249-343.
    Database link: Q7Z6M3

  • Positive control

    • WB: Mouse brain lysate; HepG2 whole cell lysate. IHC-P: Human liver cancer tissue. ICC/IF: HepG2 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HepG2 whole cell lysate (ab166833)
    • Mouse brain tissue lysate - total protein (ab30151)
    • Mouse brain tissue lysate - total protein (ab4022)

Applications

Our Abpromise guarantee covers the use of ab229951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
IHC-P 1/20 - 1/200.
WB 1/1000 - 1/5000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).

Target

  • Function

    Immunoglobulin-like receptor which plays an inhibitory role in degranulation of mast cells. Negatively regulates IgE-mediated mast cell activation and suppresses the type I immediate hypersensitivity reaction.
  • Tissue specificity

    Expressed in myeloid cells (dendritic cells, macrophages and neutrophils, weak expression on B-cells but not in T-cells or natural killer cells), peripheral blood basophils and mast cells (at protein level).
  • Sequence similarities

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession Q7Z6M3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 284021 Human
    • Entrez Gene: 380732 Mouse
    • SwissProt: Q7Z6M3 Human
    • SwissProt: Q3TB92 Mouse
    • Unigene: 631749 Human
    • Alternative names

      • Allergin-1 antibody
      • Allergy inhibitory receptor 1 antibody
      • C17orf60 antibody
      • Mast cell antigen 32 antibody
      • Mast cell immunoglobulin-like receptor 1 antibody
      • MCA-32 antibody
      • MCA32 antibody
      • MILR1 antibody
      • MILR1_HUMAN antibody
      • Probable mast cell antigen 32 homolog antibody
      see all

    Images

    • Western blot - Anti-Allergin 1 antibody (ab229951)
      Western blot - Anti-Allergin 1 antibody (ab229951)
      All lanes : Anti-Allergin 1 antibody (ab229951) at 1/1000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

      Secondary
      All lanes : Goat anti-rabbit IgG at 1/10000 dilution

      Predicted band size: 39 kDa
      Observed band size: 39 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Allergin 1 antibody (ab229951)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Allergin 1 antibody (ab229951)

      Paraffin-embedded human liver cancer tissue stained for Allergin 1 using ab229951 at 1/100 dilution in immunohistochemical analysis.

    • Immunocytochemistry/ Immunofluorescence - Anti-Allergin 1 antibody (ab229951)
      Immunocytochemistry/ Immunofluorescence - Anti-Allergin 1 antibody (ab229951)

      HepG2 (human liver hepatocellular carcinoma cell line) cell stained for Allergin 1 (green) using ab229951 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab229951? Please let us know so that we can cite the reference in this datasheet.

    ab229951 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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