Anti-Allergin 1 antibody (ab229951)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Allergin 1 antibody
See all Allergin 1 primary antibodies -
Description
Rabbit polyclonal to Allergin 1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human Allergin 1 aa 249-343.
Database link: Q7Z6M3 -
Positive control
- WB: Mouse brain lysate; HepG2 whole cell lysate. IHC-P: Human liver cancer tissue. ICC/IF: HepG2 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab229951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/50 - 1/200. | |
| IHC-P | 1/20 - 1/200. | |
| WB | 1/1000 - 1/5000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). |
Target
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Function
Immunoglobulin-like receptor which plays an inhibitory role in degranulation of mast cells. Negatively regulates IgE-mediated mast cell activation and suppresses the type I immediate hypersensitivity reaction. -
Tissue specificity
Expressed in myeloid cells (dendritic cells, macrophages and neutrophils, weak expression on B-cells but not in T-cells or natural killer cells), peripheral blood basophils and mast cells (at protein level). -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 284021 Human
- Entrez Gene: 380732 Mouse
- SwissProt: Q7Z6M3 Human
- SwissProt: Q3TB92 Mouse
- Unigene: 631749 Human
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Alternative names
- Allergin-1 antibody
- Allergy inhibitory receptor 1 antibody
- C17orf60 antibody
see all
Images
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Western blot - Anti-Allergin 1 antibody (ab229951)All lanes : Anti-Allergin 1 antibody (ab229951) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Secondary
All lanes : Goat anti-rabbit IgG at 1/10000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Allergin 1 antibody (ab229951)Paraffin-embedded human liver cancer tissue stained for Allergin 1 using ab229951 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Allergin 1 antibody (ab229951)HepG2 (human liver hepatocellular carcinoma cell line) cell stained for Allergin 1 (green) using ab229951 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
References
ab229951 has not yet been referenced specifically in any publications.
