Overview

  • Product name
    Anti-Allopregnanolone antibody
  • Description
    Rabbit polyclonal to Allopregnanolone
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Goat, Chicken, Human, Pig
  • Immunogen

    5a-PREGNAN-3a-OL-20-ONE HEMISUCCINATE conjugated to BSA

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.50
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab45164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 4 µg/ml.

Target

  • Relevance
    Allopregnanolone is a neuroactive metabolite of progesterone and a barbiturate-like modulator of central gamma-aminobutyric acid receptors that modify a range of behaviors, including the stress response. It is produced in the brain as well as glands such as the adrenals and the ovaries.

References

ab45164 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

The products are tested and guarnteed in either in direct or indirect ELISA experiments. In these types of ELISAs there is no need for a matched anti-alloprenanolone antibody as the antigen is coated on the plate itself. The anti-allopregnanolone anitbody is then detected using a secondary antibody such as Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051), Goat anti-Rabbit IgG H&L (Biotin) secondary antibody (ab97049) or Goat anti-Rabbit IgG H&L (AP) secondary antibody (ab97048) depending upon the detection substrate which you will use.

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Answer

Thank you for contacting us.

The lot numbers are actually not on the datasheet. This info is for company's internal use which can be shared with customers on case by case basis. The lot numbers are necessary to track the backward history of antibodies so these are important.

The lot with conc. 0.6mg/ml has been sold out so we sent new lot which is 1.9 mg/ml. You must be happy because you are getting 3 times more antibody in the same price.

To get working dilution just dilute the antibody and use it as recommended. e.g. for ELISA we recommend it at 4ug/ml which means you will need only 2.2 ul of antibody instead of 6.7 ul if the antibody was at 0.6mg/ml.

I am sure you will not be disappointed anymore. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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https://www.abcam.com/abreviews

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Answer

Thank you for contacting us. I am sorry for the confusion.

There are 2 different lots for this antibody, which has concentration 0.6 mg/ml and 1.900 mg/ml. The lot you have received is 914826 which has concentration of 1.9mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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