Anti-ALOX12B antibody (ab121785)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ALOX12B antibody -
Description
Rabbit polyclonal to ALOX12B -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence, corresponding to amino acids 423-516 (LYKLLIPHTR YTVQINSIGR AVLLNEGGLS AKGMSLGVEG FAGVMVRALS ELTYDSLYLP NDFVERGVQD LPGYYYRDDS LAVWNALEKY VTEI) of Human ALOX12B.
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Positive control
- Human skin tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab121785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
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| IHC-P | 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Converts arachidonic acid to 12R-hydroperoxyeicosatetraenoic acid (12R-HPETE). -
Tissue specificity
Expressed in B-cells, hair follicles, foreskin keratinocytes and adult skin. Also expressed in psoriatic tissue. -
Pathway
Lipid metabolism; leukotriene D4 biosynthesis. -
Involvement in disease
Defects in ALOX12B are a cause of non-bullous congenital ichthyosiform erythroderma (NCIE) [MIM:242100]. NCIE is a non-bullous ichthyosis, a skin disorder characterized by abnormal cornification of the epidermis. Most affected individuals are born with a tight, shiny, translucent covering called collodion membrane. The collodion membrane subsequently evolves into generalized scaling and intense redness of the skin. Clinical features are milder than in lamellar ichthyoses and demonstrate a greater variability in the intensity of erythema, size and type of scales. In contrast to lamellar ichthyoses, scales are usually white, fine and powdery, and palms and soles are severely affected. Patients suffer from palmoplantar keratoderma, often with painful fissures, digital contractures, and loss of pulp volume. -
Sequence similarities
Belongs to the lipoxygenase family.
Contains 1 lipoxygenase domain.
Contains 1 PLAT domain. - Information by UniProt
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Database links
- Entrez Gene: 242 Human
- Omim: 603741 Human
- SwissProt: O75342 Human
- Unigene: 136574 Human
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Alternative names
- 12R-lipoxygenase antibody
- 12R-LOX antibody
- 12R-type antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ALOX12B antibody (ab121785)Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab121785 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALOX12B antibody (ab121785)ab121785, at 1/10 dilution, staining ALOX12B in paraffin-embedded Human skin tissue, by Immunohistochemistry.
Datasheets and documents
References
ab121785 has not yet been referenced specifically in any publications.
