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Cardiovascular Lipids / Lipoproteins Lipid Metabolism Lipases
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Anti-ALOX12B antibody (ab121785)

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  • SDS
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Immunocytochemistry/ Immunofluorescence - Anti-ALOX12B antibody (ab121785)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALOX12B antibody (ab121785)

Key features and details

  • Rabbit polyclonal to ALOX12B
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human ALOX12B protein (ab157877)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-ALOX12B antibody
  • Description

    Rabbit polyclonal to ALOX12B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 423-516 (LYKLLIPHTR YTVQINSIGR AVLLNEGGLS AKGMSLGVEG FAGVMVRALS ELTYDSLYLP NDFVERGVQD LPGYYYRDDS LAVWNALEKY VTEI) of Human ALOX12B.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human skin tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Lipases
    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipases

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human ALOX12B protein (ab157877)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab121785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.
IHC-P
1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.
IHC-P
1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Converts arachidonic acid to 12R-hydroperoxyeicosatetraenoic acid (12R-HPETE).
  • Tissue specificity

    Expressed in B-cells, hair follicles, foreskin keratinocytes and adult skin. Also expressed in psoriatic tissue.
  • Pathway

    Lipid metabolism; leukotriene D4 biosynthesis.
  • Involvement in disease

    Defects in ALOX12B are a cause of non-bullous congenital ichthyosiform erythroderma (NCIE) [MIM:242100]. NCIE is a non-bullous ichthyosis, a skin disorder characterized by abnormal cornification of the epidermis. Most affected individuals are born with a tight, shiny, translucent covering called collodion membrane. The collodion membrane subsequently evolves into generalized scaling and intense redness of the skin. Clinical features are milder than in lamellar ichthyoses and demonstrate a greater variability in the intensity of erythema, size and type of scales. In contrast to lamellar ichthyoses, scales are usually white, fine and powdery, and palms and soles are severely affected. Patients suffer from palmoplantar keratoderma, often with painful fissures, digital contractures, and loss of pulp volume.
  • Sequence similarities

    Belongs to the lipoxygenase family.
    Contains 1 lipoxygenase domain.
    Contains 1 PLAT domain.
  • Target information above from: UniProt accession O75342 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 242 Human
    • Omim: 603741 Human
    • SwissProt: O75342 Human
    • Unigene: 136574 Human
    • Alternative names

      • 12R-lipoxygenase antibody
      • 12R-LOX antibody
      • 12R-type antibody
      • Alox12b antibody
      • Arachidonate 12-lipoxygenase antibody
      • Epidermis-type lipoxygenase 12 antibody
      • LX12B_HUMAN antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-ALOX12B antibody (ab121785)
      Immunocytochemistry/ Immunofluorescence - Anti-ALOX12B antibody (ab121785)
      Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab121785 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALOX12B antibody (ab121785)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALOX12B antibody (ab121785)
      ab121785, at 1/10 dilution, staining ALOX12B in paraffin-embedded Human skin tissue, by Immunohistochemistry.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab121785? Please let us know so that we can cite the reference in this datasheet.

    ab121785 has not yet been referenced specifically in any publications.

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