Overview

  • Product name

  • Description

    Rabbit polyclonal to ALP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (PQTVILPGPA PWGFRLSGGI DFNQPLVITR ITPGSKAAAA NLCPGDVILA) of human ALP (NP_055291).

  • Positive control

    • Fetal liver lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified by peptide affinity chromatography method.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

Target

Images

  • Anti-ALP antibody (ab83259) at 1 µg/ml (5% skim milk / PBS buffer) + fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 39 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.



    Gel concentration: 12%

References

This product has been referenced in:

  • Chen X  et al. Long non-coding RNA XIST promotes osteoporosis through inhibiting bone marrow mesenchymal stem cell differentiation. Exp Ther Med 17:803-811 (2019). Read more (PubMed: 30651866) »
  • Huang B  et al. Amifostine Suppresses the Side Effects of Radiation on BMSCs by Promoting Cell Proliferation and Reducing ROS Production. Stem Cells Int 2019:8749090 (2019). Read more (PubMed: 30728842) »
See all 11 Publications for this product

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