Product nameAnti-ALP antibody
See all ALP primary antibodies
DescriptionRabbit polyclonal to ALP
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
- Fetal liver lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified by peptide affinity chromatography method.
Our Abpromise guarantee covers the use of ab83259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/1562500.
FunctionMay play a role in the organization of actin filament arrays within muscle cells.
Tissue specificityIsoform 1 is highly expressed in differentiated skeletal muscle. Isoform 2 is heart-specific.
Sequence similaritiesContains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain.
Cellular localizationCytoplasm > myofibril > sarcomere > Z line. Localizes to myofiber Z-lines.
- Information by UniProt
- Actinin associated LIM protein antibody
- Actinin-associated LIM protein antibody
- Alpha actinin 2 associated LIM protein antibody
Anti-ALP antibody (ab83259) at 1 µg/ml (5% skim milk / PBS buffer) + fetal liver lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
This product has been referenced in:
- Chen X et al. Long non-coding RNA XIST promotes osteoporosis through inhibiting bone marrow mesenchymal stem cell differentiation. Exp Ther Med 17:803-811 (2019). Read more (PubMed: 30651866) »
- Huang B et al. Amifostine Suppresses the Side Effects of Radiation on BMSCs by Promoting Cell Proliferation and Reducing ROS Production. Stem Cells Int 2019:8749090 (2019). Read more (PubMed: 30728842) »