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Neuroscience Neurology process Neurodegenerative disease Parkinson's disease Synuclein
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RecombinantRabMAb

Recombinant Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (14)

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Immunocytochemistry/ Immunofluorescence - Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252)
  • Flow Cytometry - Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252)
  • Western blot - Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252)
  • Western blot - Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252)
  • Flow Cytometry - Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252)
  • Western blot - Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252)
  • Flow Cytometry - Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252)
  • Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1646Y] to alpha + beta Synuclein
  • Suitable for: Flow Cyt, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-alpha + beta Synuclein antibody [EP1646Y]
    See all alpha + beta Synuclein primary antibodies
  • Description

    Rabbit monoclonal [EP1646Y] to alpha + beta Synuclein
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Rat
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human alpha + beta Synuclein aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Human fetal brain, human cerebellum, mouse brain and rat brain tissue lysates. ICC/IF: PC12 and rat primary hippocampal neuron cells. Flow Cyt: SH-SY5Y and U87-MG cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1646Y
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Synuclein
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Tangles & Tau
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other

Associated products

  • Alternative Versions

    • Anti-alpha + beta Synuclein antibody [EP1646Y] - BSA and Azide free (ab189217)
    • Alexa Fluor® 488 Anti-alpha + beta Synuclein antibody [EP1646Y] (ab199086)
    • Alexa Fluor® 647 Anti-alpha + beta Synuclein antibody [EP1646Y] (ab199304)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Mouse brain tissue lysate - total protein (ab30151)
    • Mouse brain tissue lysate - total protein (0 days) (ab7188)
    • Mouse brain tissue lysate - total protein (14 days) (ab7189)
    • Mouse brain tissue lysate - total protein (7 days) (ab7190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab51252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Rat
WB
Mouse
Human
Application Abreviews Notes
Flow Cyt
1/30.

For unpurified use at 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/5000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).

For unpurified use at 1/50000.

ICC/IF (1)
1/50 - 1/100.
Notes
Flow Cyt
1/30.

For unpurified use at 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/5000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).

For unpurified use at 1/50000.

ICC/IF
1/50 - 1/100.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Relevance

      Synucleins are small soluble proteins expressed primarily in neural tissues and in certain tumors. The family includes 3 known proteins, alpha synuclein, beta synuclein and gamma synuclein. All synucleins have in common a highly conserved alpha helical lipid binding motif with similarity to the class A2 lipid binding domains of the exchangeable apolipoproteins. The alpha and beta synuclein proteins are found primarily in brain tissue, where they are seen mainly in pre synaptic terminals. Alpha synuclein is believed to be a major component of Lewy bodies in Parkinson’s disease. Mutations in alpha synuclein are associated with rare familial cases of early onset Parkinson’s disease, Alzheimer’s disease and several other neurodegenerative illnesses.
    • Cellular localization

      Cytoplasmic. Membrane bound in dopaminergic neurons. Also found in the nucleus.
    • Database links

      • Entrez Gene: 6620 Human
      • Entrez Gene: 6622 Human
      • Entrez Gene: 104069 Mouse
      • Entrez Gene: 20617 Mouse
      • Entrez Gene: 113893 Rat
      • Entrez Gene: 29219 Rat
      • Omim: 163890 Human
      • Omim: 602569 Human
      • SwissProt: P37840 Human
      • SwissProt: Q16143 Human
      • SwissProt: O55042 Mouse
      • SwissProt: Q91ZZ3 Mouse
      • SwissProt: P37377 Rat
      • SwissProt: Q63754 Rat
      • Unigene: 271771 Human
      • Unigene: 90297 Human
      • Unigene: 17484 Mouse
      • Unigene: 200843 Mouse
      • Unigene: 1827 Rat
      • Unigene: 20352 Rat
      see all
    • Alternative names

      • Alpha synuclein antibody
      • Beta synuclein antibody
      • NACP antibody
      • Non A beta component of AD amyloid antibody
      • PARK1 antibody
      • PARK4 antibody
      • PD1 antibody
      • SNCA antibody
      • SNCB antibody
      • Synuclein alpha antibody
      • Synuclein beta antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252)
      Immunocytochemistry/ Immunofluorescence - Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252)

      Immunocytochemistry/Immunofluorescence analysis of PC-12 cells labelling alpha + beta Synuclein with purified ab51252 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

      Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

    • Flow Cytometry - Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252)
      Flow Cytometry - Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252)
      Overlay histogram showing U87-MG cells fixed in 4% PFA and stained with purified ab51252 at a dilution of 1 in 30 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).
    • Western blot - Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252)
      Western blot - Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252)
      All lanes : Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252) at 1/5000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 14 kDa
      Observed band size: 18 kDa
      why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM /TBST.

    • Western blot - Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252)
      Western blot - Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252)
      Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252) at 1/5000 dilution (purified) + Human cerebellum tissue lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 14 kDa
      Observed band size: 18 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM /TBST.

    • Flow Cytometry - Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252)
      Flow Cytometry - Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252)

      Flow Cytometry analysis of U87-MG cells labelling alpha + beta Synuclein with purified ab51252 at 1/30 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    • Western blot - Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252)
      Western blot - Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252)
      Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252) at 1/50000 dilution (unpurified) + Human fetal brain tissue lysate at 10 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 14 kDa
      Observed band size: 18 kDa why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252)
      Immunocytochemistry/ Immunofluorescence - Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252)Image from Hejjaoui M et al., J Am Chem Soc. 2012 Mar 21;134(11):5196-210. Epub 2012 Mar 13. Fig 4.; DOI: 10.1021/ja210866j; February 16, 2012, J. Am. Chem. Soc., 2012, 134 (11), pp 5196–5210, with permission from the American Chemical Society.

      Immunocytochemistry/Immunofluorescence analysis of rat primary hippocampal neurons, staining alpha + beta Synuclein with unpurified ab51252. Cells were fixed, permeabilized, and blocked with 10% donkey serum at room temperature. Cells were incubated with primary antibody (1/1000) at 4°C for 24 hours. A Cy2-conjugated donkey anti-rabbit IgG was used as the secondary antibody.

    • Flow Cytometry - Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252)
      Flow Cytometry - Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252)

      Overlay histogram showing SH-SY5Y cells stained with unpurified ab51252 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified ab51252, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    • Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252)
      Anti-alpha + beta Synuclein antibody [EP1646Y] (ab51252)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (14)

    Publishing research using ab51252? Please let us know so that we can cite the reference in this datasheet.

    ab51252 has been referenced in 14 publications.

    • Stoyka LE  et al. Behavioral defects associated with amygdala and cortical dysfunction in mice with seeded a-synuclein inclusions. Neurobiol Dis 134:104708 (2020). PubMed: 31837424
    • McGlinchey RP  et al. C-terminal a-synuclein truncations are linked to cysteine cathepsin activity in Parkinson's disease. J Biol Chem 294:9973-9984 (2019). PubMed: 31092553
    • Even A  et al. ATAT1-enriched vesicles promote microtubule acetylation via axonal transport. Sci Adv 5:eaax2705 (2019). PubMed: 31897425
    • Nash Y  et al. DJ-1 deficiency impairs autophagy and reduces alpha-synuclein phagocytosis by microglia. J Neurochem 143:584-594 (2017). PubMed: 28921554
    • Binolfi A  et al. Intracellular repair of oxidation-damaged a-synuclein fails to target C-terminal modification sites. Nat Commun 7:10251 (2016). WB, ICC/IF . PubMed: 26807843
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha + beta Synuclein antibody [EP1646Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Sample
    Human Cell (SH-SY5Y)
    Specification
    SH-SY5Y
    Permeabilization
    Yes - 0.5% Tween
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 03 2014

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