Overview

  • Product name

    Anti-alpha 1 Sodium Potassium ATPase antibody
    See all alpha 1 Sodium Potassium ATPase primary antibodies
  • Description

    Rabbit polyclonal to alpha 1 Sodium Potassium ATPase
  • Host species

    Rabbit
  • Specificity

    Ab33133 recognises alpha 1 subunit of Sodium Potassium ATPase.
  • Tested applications

    Suitable for: IP, IHC-Frmore details
  • Species reactivity

    Reacts with: Rat, Cow
    Does not react with: Rabbit
  • Immunogen

    Fusion protein, corresponding to amino acids 338-518 of Rat alpha 1 Sodium Potassium ATPase.

  • Positive control

    • WB: Rat kidney microsomes. IP: Rat kidney and brai microsomes. ICC/IHC-Fr: Rat kidney and brain.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.184% Tris glycine, 40% Glycerol, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 4 µg/ml. Use 4 µg to immunoprecipate the a 1 subunit from 500 µg rat kidney microsomes.
IHC-Fr 1/5. Use at a concentration of 5 µg/ml to detect the a 1 subunit in frozen sections of rat kidney and brain, which were fixed in 4% paraformaldehyde.

Target

  • Function

    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
  • Sequence similarities

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
  • Post-translational
    modifications

    Phosphorylation on Tyr-10 modulates pumping activity.
  • Cellular localization

    Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • A1A1 antibody
    • AT1A1 antibody
    • AT1A1_HUMAN antibody
    • ATP1A1 antibody
    • Atpa-1 antibody
    • ATPase Na+/K+ transporting alpha 1 polypeptide antibody
    • ATPase Na+/K+ transporting subunit alpha 1 antibody
    • BC010319 antibody
    • EC 3.6.3.9 antibody
    • MGC3285 antibody
    • MGC38419 antibody
    • MGC51750 antibody
    • Na K ATPase alpha A catalytic polypeptide antibody
    • Na K ATPase catalytic subunit alpha A protein antibody
    • Na(+)/K(+) ATPase 1 antibody
    • Na(+)/K(+) ATPase alpha-1 subunit antibody
    • Na+, K+ ATPase alpha subunit antibody
    • Na+/K+ ATPase alpha 1 subunit antibody
    • Na+/K+ ATPase 1 antibody
    • Na,K ATPase alpha 1 subunit antibody
    • Nkaa1b antibody
    • Sodium potassium ATPase alpha 1 polypeptide antibody
    • Sodium pump 1 antibody
    • Sodium pump subunit alpha-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-1 antibody
    • Sodium/potassium-transporting ATPase subunit alpha-1 antibody
    see all

References

This product has been referenced in:

  • Mahendrasingam S  et al. Subcellular distribution and relative expression of fibrocyte markers in the CD/1 mouse cochlea assessed by semiquantitative immunogold electron microscopy. J Histochem Cytochem 59:984-1000 (2011). Read more (PubMed: 22043022) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.

There are several antibodies in our catalogue targeting either the alpha or beta subunit of Sodium Potassium ATPase.

I have conducted a search to find the most suitable for your experiment, and the best options are linked below:

https://www.abcam.com/alpha-3-Sodium-Potassium-ATPase-antibody-ab78798.html (only guaranteed in paraffin embedded tissue; targets the alpha 3 subunit)

https://www.abcam.com/alpha-1-Sodium-Potassium-ATPase-phospho-S23-antibody-ab74069.html (only tested with immunocytochemistry; specific to the alpha 1subnunit)

https://www.abcam.com/beta-Sodium-Potassium-ATPase-antibody-0-T-05-ab33144.html (only characterised by IHC-P; targets the beta 1 subunit)

https://www.abcam.com/alpha-1-Sodium-Potassium-ATPase-antibody-ab33133.html (recognises alpha 1 subunit of Sodium Potassium ATPase and is suitable for frozen sections).

I hope this information is helpful to you. If you have specific questions about the products identified during the search, please do not hesitate to contact me.

Read More

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