Product nameAlpha 1 Acid Glycoprotein overexpression 293T lysate (whole cell)
ab94216 is a 293T cell transfected lysate in which Human Alpha 1 Acid Glycoprotein has been transiently over-expressed using a pCMV-Alpha 1 Acid Glycoprotein plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: Appears to function in modulating the activity of the immune system during the acute-phase reaction. Tissue specificity: Expressed by the liver and secreted in plasma. Similarity: Belongs to the calycin superfamily. Lipocalin family.
Our Abpromise guarantee covers the use of ab94216 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94216 at 15μg/lane on an SDS-PAGE gel.
All lanes : Anti-Alpha 1 Acid Glycoprotein/AGP antibody (ab58291) at 1/500 dilution
Lane 1 : Alpha 1 Acid Glycoprotein 293T Transfected Lysate - Positive Control (ab94216)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution
ab94216 has not yet been referenced specifically in any publications.