Western blot abreview for Anti-alpha 1 Adrenergic Receptor/ADRA1 antibody

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - other (cellular fractionation of hindpaw skin protein)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
cellular fractionation of hindpaw skin protein
Blocking step
LiCOR blocking buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 24°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Dilution
1/400

Secondary antibody

Dilution
1/50000
Name
Non-Abcam antibody was used: LiCOR IrDye680 goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680RD

Detection

Detection method
Odyssey fluorescent imaging system
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: 55 kDa
Positive control
None

Additional data

Additional Notes
cyt= cytoplasmic, mem=membrane, cbnuc=chromatin-bound, cytsk=cytoskeletal

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Submitted Sep 30 2015

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