Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-alpha 1 Adrenergic Receptor/ADRA1 antibody

Average
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
Yes - 0.2% Triton X-100
Specification
heart
Blocking step
Serum as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde

Other product details

Incubation time
10 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.05% Serum+ 0.01% Triton in PBS
Dilution
1/150

Secondary antibody

Dilution
1/300
Name
Non-Abcam antibody was used: Anti Rabbit 488
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

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Submitted Nov 01 2016

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