Recombinant
RabMAb

Recombinant Anti-alpha 1 Adrenergic Receptor/ADRA1 antibody [EPR11821(2)] (ab192614)

Overview

  • Product name

    Anti-alpha 1 Adrenergic Receptor/ADRA1 antibody [EPR11821(2)]
    See all alpha 1 Adrenergic Receptor/ADRA1 primary antibodies
  • Description

    Rabbit monoclonal [EPR11821(2)] to alpha 1 Adrenergic Receptor/ADRA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human alpha 1 Adrenergic Receptor/ADRA1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P25100

  • Positive control

    • Raji, RAW 264.7, NIH 3T3 and Human fetal heart lysates.
  • General notes

     

     

     This product was previously labelled as alpha 1 Adrenergic Receptor, alpha 1d Adrenergic Receptor

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab192614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
IP 1/50.

Target

Images

  • Western blot analysis of immunoprecipitation pellet from Raji cell lysate immunoprecipitated using ab192614 at 1/50 dilution (lane 1) or PBS control (lane 2).
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • All lanes : Anti-alpha 1 Adrenergic Receptor/ADRA1 antibody [EPR11821(2)] (ab192614) at 1/1000 dilution

    Lane 1 : RAW 264.7 cell lysate
    Lane 2 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 60 kDa

  • All lanes : Anti-alpha 1 Adrenergic Receptor/ADRA1 antibody [EPR11821(2)] (ab192614) at 1/1000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : Human fetal heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 60 kDa

References

This product has been referenced in:

  • Ha SH  et al. Induction of GD3/a1-adrenergic receptor/transglutaminase 2-mediated erythroid differentiation in chronic myelogenous leukemic K562 cells. Oncotarget 8:72205-72219 (2017). Read more (PubMed: 29069780) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (Brain, dorsal lateral pons)
Specification
Brain, dorsal lateral pons

Abcam user community

Verified customer

Submitted Aug 14 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up