Anti-AACT antibody (ab9374)
Key features and details
- Rabbit polyclonal to AACT
- Suitable for: IHC-P, Sandwich ELISA, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AACT antibody
See all AACT primary antibodies -
Description
Rabbit polyclonal to AACT -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Sandwich ELISA, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: MammalsDoes not react with: Mouse -
Immunogen
Full length native protein (purified) corresponding to AACT. Alpha-1-antichymotrypsin isolated from human serum.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Constituents: PBS, 0.01425% Magnesium chloride, 0.00154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.01% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9374 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/25 - 1/50. ABC method.
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Sandwich ELISA |
Use at an assay dependent concentration.
For sandwich ELISA, use this antibody as Detection at an assay dependent concentration with Mouse monoclonal to AACT (ab54693) as Capture. |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 48 kDa).
Assay Dependent |
Notes |
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IHC-P
1/25 - 1/50. ABC method. |
Sandwich ELISA
Use at an assay dependent concentration. For sandwich ELISA, use this antibody as Detection at an assay dependent concentration with Mouse monoclonal to AACT (ab54693) as Capture. |
WB
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 48 kDa). Assay Dependent |
Target
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Function
Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2. -
Tissue specificity
Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains. -
Involvement in disease
Defects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280]. -
Sequence similarities
Belongs to the serpin family. -
Domain
The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 12 Human
- Omim: 107280 Human
- SwissProt: P01011 Human
- Unigene: 534293 Human
- Unigene: 710488 Human
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Alternative names
- SERPINA3 antibody
- AACT antibody
- AACT_HUMAN antibody
see all
Images
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All lanes : Anti-AACT antibody (ab9374) at 1 µg/ml
Lane 1 : Human spinal cord tissue lysate - total protein (ab29188)
Lane 2 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 48 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted? -
Standard Curve for AACT (Analyte: AACT protein (Human) (ab80517) ); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to AACT (ab54693) at 5µg/ml and Detector Antibody Rabbit polyclonal to AACT (ab9374) at 0.5µg/ml.
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Human normal skin. Staining is mainly membrane-bound and cytoplasmic. Left panel: with primary antibody at 2 ug/ml. Right panel: negative control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for rabbit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab9374 has been referenced in 1 publication.
- Martin-Rojas T et al. A clinical perspective on the utility of alpha 1 antichymotrypsin for the early diagnosis of calcific aortic stenosis. Clin Proteomics 14:12 (2017). WB, IHC . PubMed: 28439213