Overview

  • Product name

    Anti-alpha 1 Antitrypsin antibody [11G2]
    See all alpha 1 Antitrypsin primary antibodies
  • Description

    Mouse monoclonal [11G2] to alpha 1 Antitrypsin
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Dog, Human, Monkey
  • Immunogen

    Recombinant full length protein corresponding to Human alpha 1 Antitrypsin. (Expressed in HEK-293T cells).
    Database link: P01009

  • Positive control

    • IHC-P: Human liver tissue. WB: HEK-293T cell lysate transfected with pCMV6-ENTRY alpha 1 Antitrypsin; HepG2, HeLa, HT-29, COS-7, Jurkat, MDCK and MCF7 cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab230695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/2000. Predicted molecular weight: 46 kDa.
IP Use at an assay dependent concentration.

Target

  • Function

    Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
    Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
  • Tissue specificity

    Ubiquitous. Expressed in leukocytes and plasma.
  • Involvement in disease

    Alpha-1-antitrypsin deficiency
  • Sequence similarities

    Belongs to the serpin family.
  • Domain

    The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • Post-translational
    modifications

    N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
    Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
  • Cellular localization

    Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • A1A antibody
    • A1AT antibody
    • A1AT_HUMAN antibody
    • AAT antibody
    • Alpha 1 antiproteinase antibody
    • Alpha 1 antitrypsin antibody
    • Alpha 1 antitrypsin null antibody
    • Alpha 1 protease inhibitor antibody
    • Alpha-1 protease inhibitor antibody
    • Alpha-1-antiproteinase antibody
    • alpha1 proteinase inhibitor antibody
    • Alpha1AT antibody
    • Dom1 antibody
    • PI antibody
    • PI1 antibody
    • PRO0684 antibody
    • PRO2275 antibody
    • Serine (or cysteine) proteinase inhibitor clade A member 1 antibody
    • Serine protease inhibitor 1-1 antibody
    • Serine protease inhibitor A1a antibody
    • Serpin A1 antibody
    • Serpin A1a antibody
    • Serpin peptidase inhibitor clade A member 1 antibody
    • Serpina1 antibody
    • Short peptide from AAT antibody
    • SPAAT antibody
    • Spi1-1 antibody
    see all

Images

  • All lanes : Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY alpha 1 Antitrypsin

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 46 kDa

  • Formalin-fixed, paraffin-embedded human liver tissue stained for alpha 1 Antitrypsin using ab230695 at 10 µg/ml in immunohistochemical analysis.

  • All lanes : Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695) at 1/1000 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
    Lane 3 : HT-29 (human colorectal adenocarcinoma cell line) cell extract
    Lane 4 : A549 (human lung carcinoma cell line) cell extract
    Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell extract
    Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
    Lane 7 : MDCK (canine kidney cell line) cell extract
    Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract
    Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell extract

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 46 kDa

  • alpha 1 Antitrypsin was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) DDK tagged alpha 1 Antitrypsin antibody overexpression lysate with 2 µg ab230695 and 20 µl (0.1 mg) of goat anti-mouse conjugated magnetic beads. Western blot was performed from the immunoprecipitate using rabbit anti-DDK polyclonal antibody.

    Lane 1: Negative control: IP without adding anti-alpha 1 Antitrypsin antibody in HEK-293T DDK tagged alpha 1 Antitrypsin antibody overexpression lysate.

    Lane 2: ab230695 IP in -293T DDK tagged alpha 1 Antitrypsin antibody overexpression lysate.

References

ab230695 has not yet been referenced specifically in any publications.

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