Key features and details
- Mouse monoclonal [AAT/1378] to alpha 1 Antitrypsin
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-alpha 1 Antitrypsin antibody [AAT/1378]
See all alpha 1 Antitrypsin primary antibodies
DescriptionMouse monoclonal [AAT/1378] to alpha 1 Antitrypsin
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human alpha 1 Antitrypsin. The exact sequence is proprietary.
Database link: P01009
- Human tonsil tissue, recombinant alpha 1 Antitrypsin, Jurkat or A549 whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from bioreactor concentrates
Our Abpromise guarantee covers the use of ab218478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 46 kDa.|
FunctionInhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
Tissue specificityUbiquitous. Expressed in leukocytes and plasma.
Involvement in diseaseAlpha-1-antitrypsin deficiency
Sequence similaritiesBelongs to the serpin family.
DomainThe reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
modificationsN-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
Cellular localizationSecreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix.
- Information by UniProt
- A1A antibody
- A1AT antibody
- A1AT_HUMAN antibody
All lanes : Anti-alpha 1 Antitrypsin antibody [AAT/1378] (ab218478) at 1 µg/ml
Lane 1 : Recombinant alpha 1 Antitrypsin
Lane 2 : Jurkat whole cell lysate
Lane 3 : A549 whole cell lysate
Predicted band size: 46 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded section) of Human tonsil tissue labeling alpha 1 Antitrypsin with ab218478 at 1ug/ml.
ab218478 has not yet been referenced specifically in any publications.