Overview

  • Product name

    Anti-alpha 1 Antitrypsin antibody
    See all alpha 1 Antitrypsin primary antibodies
  • Description

    Rabbit polyclonal to alpha 1 Antitrypsin
  • Host species

    Rabbit
  • Specificity

    The antibody is specific against human alpha-1-antitrypsin.
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mammals
  • Immunogen

    Alpha-1-antitrypsin isolated from human serum.

  • Positive control

    • IHC-P: Human tonsil tissue. ICC/IF: HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab9373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 1 µg/ml.

Target

  • Function

    Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
    Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
  • Tissue specificity

    Ubiquitous. Expressed in leukocytes and plasma.
  • Involvement in disease

    Alpha-1-antitrypsin deficiency
  • Sequence similarities

    Belongs to the serpin family.
  • Domain

    The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • Post-translational
    modifications

    N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
    Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
  • Cellular localization

    Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • A1A antibody
    • A1AT antibody
    • A1AT_HUMAN antibody
    • AAT antibody
    • Alpha 1 antiproteinase antibody
    • Alpha 1 antitrypsin antibody
    • Alpha 1 antitrypsin null antibody
    • Alpha 1 protease inhibitor antibody
    • Alpha-1 protease inhibitor antibody
    • Alpha-1-antiproteinase antibody
    • alpha1 proteinase inhibitor antibody
    • Alpha1AT antibody
    • Dom1 antibody
    • PI antibody
    • PI1 antibody
    • PRO0684 antibody
    • PRO2275 antibody
    • Serine (or cysteine) proteinase inhibitor clade A member 1 antibody
    • Serine protease inhibitor 1-1 antibody
    • Serine protease inhibitor A1a antibody
    • Serpin A1 antibody
    • Serpin A1a antibody
    • Serpin peptidase inhibitor clade A member 1 antibody
    • Serpina1 antibody
    • Short peptide from AAT antibody
    • SPAAT antibody
    • Spi1-1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling alpha 1 Antitrypsin with ab9373.

  • ICC/IF image of ab9373 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9373, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab9373 staining alpha 1 Antitrypsin in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry.

References

This product has been referenced in:

  • Smith SM & Melrose J A Retrospective Analysis of the Cartilage Kunitz Protease Inhibitory Proteins Identifies These as Members of the Inter-a-Trypsin Inhibitor Superfamily with Potential Roles in the Protection of the Articulatory Surface. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30678366) »
  • Skurikhin EG  et al. Endothelial Progenitor Cells as Pathogenetic and Diagnostic Factors, and Potential Targets for GLP-1 in Combination with Metabolic Syndrome and Chronic Obstructive Pulmonary Disease. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30836679) »
See all 8 Publications for this product

Customer reviews and Q&As

Filter by Application

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1-5 of 5 Abreviews

Application
Immunoprecipitation
Sample
Human Tissue lysate - other (Synovial fluid)
Total protein in input
500 µg
Immuno-precipitation step
Other - Agarose amine-reactive support
Specification
Synovial fluid

Dr. Tania Colasanti

Verified customer

Submitted Jun 03 2016

Application
Western blot
Sample
Human Tissue lysate - other (Synovial fluid)
Gel Running Conditions
Reduced Denaturing (7% gel SDS PAGE)
Loading amount
2 µg
Specification
Synovial fluid
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Dr. Tania Colasanti

Verified customer

Submitted May 23 2016

Application
ELISA
Sample
Human Purified protein (Native)
Specification
Native
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
Type
Indirect

Dr. Tania Colasanti

Verified customer

Submitted May 23 2016

Application
ELISA
Sample
Rabbit Purified protein (purified protein)
Specification
purified protein
Type
Sandwich (Capture)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.25% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Feb 12 2014

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (liver)
Specification
liver
Fixative
Acetone
Permeabilization
No
Blocking step
GTx30972 as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Dec 21 2012

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