Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha 1 Antitrypsin antibody [EPR17087-50]

Inconclusive
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
Permeabilization
No
Specification
Liver
Blocking step
PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde

Other product details

Incubation time
30 minute(s) · Diluent: Leica Bond Diluent
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Dako Envision Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
No staining visible in mouse liver sections.
Abcam response
Thank you for taking part in our AbTrial program and your honest feedback. As the product had not yet been tested in this species we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in mouse.

Abcam user community

Verified customer

Submitted Feb 07 2018

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