RecombinantRabMAb

Recombinant Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)

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Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)
  • Western blot - Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)
  • Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9090] to alpha 1 Antitrypsin
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-alpha 1 Antitrypsin antibody [EPR9090]
    See all alpha 1 Antitrypsin primary antibodies

  • Description

    Rabbit monoclonal [EPR9090] to alpha 1 Antitrypsin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment corresponding to Human alpha 1 Antitrypsin.

  • Positive control

    • WB: Human serum and fetal liver lysates. ICC/IF: HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab166610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 46 kDa).
ICC/IF 1/100.
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
      Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).

    • Tissue specificity

      Ubiquitous. Expressed in leukocytes and plasma.

    • Involvement in disease

      Alpha-1-antitrypsin deficiency

    • Sequence similarities

      Belongs to the serpin family.

    • Domain

      The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.

    • Post-translational
      modifications

      N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
      Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.

    • Cellular localization

      Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix.
    • Information by UniProt

    • Database links

      • Alternative names

        • A1A antibody
        • A1AT antibody
        • A1AT_HUMAN antibody
        • AAT antibody
        • Alpha 1 antiproteinase antibody
        • Alpha 1 antitrypsin antibody
        • Alpha 1 antitrypsin null antibody
        • Alpha 1 protease inhibitor antibody
        • Alpha-1 protease inhibitor antibody
        • Alpha-1-antiproteinase antibody
        • alpha1 proteinase inhibitor antibody
        • Alpha1AT antibody
        • Dom1 antibody
        • PI antibody
        • PI1 antibody
        • PRO0684 antibody
        • PRO2275 antibody
        • Serine (or cysteine) proteinase inhibitor clade A member 1 antibody
        • Serine protease inhibitor 1-1 antibody
        • Serine protease inhibitor A1a antibody
        • Serpin A1 antibody
        • Serpin A1a antibody
        • Serpin peptidase inhibitor clade A member 1 antibody
        • Serpina1 antibody
        • Short peptide from AAT antibody
        • SPAAT antibody
        • Spi1-1 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)
        Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)

        ab166610 stained HepG2 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab166610 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

      • Western blot - Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)
        Western blot - Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)
        All lanes : Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610) at 1/1000 dilution

        Lane 1 : Human serum lysate
        Lane 2 : Fetal liver lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 46 kDa
        Observed band size: 52 kDa
        why is the actual band size different from the predicted?

      • Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)
        Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)

      References (1)

      Publishing research using ab166610? Please let us know so that we can cite the reference in this datasheet.

      ab166610 has been referenced in 1 publication.

      • Zhou T  et al. Alpha-1 Antitrypsin Attenuates M1 Microglia-Mediated Neuroinflammation in Retinal Degeneration. Front Immunol 9:1202 (2018). PubMed: 29899745

      Customer reviews and Q&As

      Show All Reviews Q&A
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      Western blot abreview for Anti-alpha 1 Antitrypsin antibody [EPR9090]

       Average
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Serum (serum (loading in microliter per lane))
      Gel Running Conditions
      Reduced Denaturing (12 %)
      Loading amount
      2 µg
      Specification
      serum (loading in microliter per lane)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
      Read More

      Georg Haase

      Verified customer

      Submitted Feb 08 2016

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