Recombinant Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-alpha 1 Antitrypsin antibody [EPR9090]
See all alpha 1 Antitrypsin primary antibodies -
Description
Rabbit monoclonal [EPR9090] to alpha 1 Antitrypsin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human alpha 1 Antitrypsin.
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Positive control
- WB: Human serum and fetal liver lysates. ICC/IF: HepG2 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9090 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-alpha 1 Antitrypsin antibody [EPR9090-71] (ab179443)
- Anti-alpha 1 Antitrypsin antibody [EPR9090] (Alexa Fluor® 488) (ab206734)
- Anti-alpha 1 Antitrypsin antibody [EPR9090] (Alexa Fluor® 647) (ab206735)
- Anti-alpha 1 Antitrypsin antibody [EPR9090] - BSA and Azide free (ab249351)
- Anti-alpha 1 Antitrypsin antibody [EPR9090-71] - BSA and Azide free (ab250088)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab166610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 46 kDa). | |
| ICC/IF | 1/100. |
Target
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Function
Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). -
Tissue specificity
Ubiquitous. Expressed in leukocytes and plasma. -
Involvement in disease
Alpha-1-antitrypsin deficiency -
Sequence similarities
Belongs to the serpin family. -
Domain
The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable. -
Post-translational
modificationsN-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. -
Cellular localization
Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 5265 Human
- Entrez Gene: 24648 Rat
- Omim: 107400 Human
- SwissProt: P01009 Human
- SwissProt: P17475 Rat
- Unigene: 525557 Human
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Alternative names
- A1A antibody
- A1AT antibody
- A1AT_HUMAN antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)](https://www.abcam.com/ps/products/166/ab166610/Images/ab166610-236055-anti-alpha-1-antitrypsin-antibody-epr9090-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)ab166610 stained HepG2 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab166610 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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![Western blot - Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)](https://www.abcam.com/ps/products/166/ab166610/Images/ab166610-164835-anti-alpha-1-antitrypsin-antibody-epr9090-western-blot.jpg)
Western blot - Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)All lanes : Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610) at 1/1000 dilution
Lane 1 : Human serum lysate
Lane 2 : Fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 46 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Datasheets and documents
References
This product has been referenced in:
- Zhou T et al. Alpha-1 Antitrypsin Attenuates M1 Microglia-Mediated Neuroinflammation in Retinal Degeneration. Front Immunol 9:1202 (2018). Read more (PubMed: 29899745) »
