Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-alpha 1 Antitrypsin antibody [EPR9090]
See all alpha 1 Antitrypsin primary antibodies -
DescriptionRabbit monoclonal [EPR9090] to alpha 1 Antitrypsin
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Recombinant fragment corresponding to a region of Human alpha 1 Antitrypsin (UniProt ID: P01009).
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Positive control
- WB: Human serum and fetal liver lysates. ICC/IF: HepG2 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR9090
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab166610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 46 kDa). | |
| ICC/IF | 1/100. |
Target
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FunctionInhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). -
Tissue specificityUbiquitous. Expressed in leukocytes and plasma.
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Involvement in diseaseAlpha-1-antitrypsin deficiency
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Sequence similaritiesBelongs to the serpin family.
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DomainThe reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
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Post-translational
modificationsN-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. -
Cellular localizationSecreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix.
- Information by UniProt
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Database links
- Entrez Gene: 5265 Human
- Entrez Gene: 24648 Rat
- Omim: 107400 Human
- SwissProt: P01009 Human
- SwissProt: P17475 Rat
- Unigene: 525557 Human
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Alternative names
- A1A antibody
- A1AT antibody
- A1AT_HUMAN antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)](https://www.abcam.com/ps/products/166/ab166610/Images/ab166610-236055-ab166610hepg2m1in100edit.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)ab166610 stained HepG2 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab166610 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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![Western blot - Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)](https://www.abcam.com/ps/products/166/ab166610/Images/alpha-1-Antitrypsin-Primary-antibodies-ab166610-1.jpg)
Western blot - Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610)All lanes : Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610) at 1/1000 dilution
Lane 1 : Human serum lysate
Lane 2 : Fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 46 kDa
Observed band size: 52 kDa (why is the actual band size different from the predicted?)
References
ab166610 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"