For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
For the following products :
Anti-SerpinB9 antibody [7D8] (ab46533)
Anti-Antithrombin III antibody [8D5] (ab117851)
Anti-alpha 1 Antitrypsin antibody (FITC) (ab19170)
I would like to know if :
- we could use them in flow cytometry analysis ?
- we need to perform intracellular staining and thus use FIX/PERM steps ?
- we need to use Protein Transport Inhibitor and what abcam suggests .
- you could send me a respective protocol ?
Asked on Nov 15 2012
Thank you for contacting us.
Ab19170, ab46533 and ab117851 have all been validated in Flow Cyt and therefore are guaranteed to work in this application.
Regarding ab19170, cells were were fixed with 4% paraformaldehyde but not permeabilised. Please look at the following publication for the protocol used:
Bergin DA et al. a-1 Antitrypsin regulates human neutrophil chemotaxis induced by soluble immune complexes and IL-8. J Clin Invest 120:4236-50 (2010).
Regarding ab46533, we recommend that a permeabilisation step be performed in order to stain with this antibody. Our labs did not use any kind of inhibitor in their staining and our suggested protocol is attached.
Regarding ab117851, we recommend FIX/PERM steps in order to stain with this antibody (see protocol also attached.)
Protein Transport Inhibitor is normally applied only during the final hours of the stimulation protocol. So it shouldn't affect the whole FACS protocol.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
Answered on Nov 15 2012