• Product name

    Anti-alpha 1 Antitrypsin antibody (HRP)
    See all alpha 1 Antitrypsin primary antibodies
  • Description

    Sheep polyclonal to alpha 1 Antitrypsin (HRP)
  • Host species

  • Conjugation

  • Tested applications

    Suitable for: ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Human α1-antitrypsin purified from human plasma and shown to be pure by IEP.

  • General notes

    Horseradish peroxidase by the periodate method



Our Abpromise guarantee covers the use of ab8768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ELISA: To give an A450nm = 1.0 using wells coated with human a1-antitrypsin at 5µg/mL andtetramethylbenzidine as the chromogen: 1/6400 – 1/12800
    The titres reported should be used as guidelines for determining the working dilution in the users system.
    The addition of Tween 20 (0.05 – 0.1%) to the conjugate diluent buffer is recommended to prevent non-specific binding in ELISA.
    It may also be advantageous in other assay systems.
  • Target

    • Function

      Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
      Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
    • Tissue specificity

      Ubiquitous. Expressed in leukocytes and plasma.
    • Involvement in disease

      Alpha-1-antitrypsin deficiency
    • Sequence similarities

      Belongs to the serpin family.
    • Domain

      The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
    • Post-translational

      N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
      Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
    • Cellular localization

      Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • A1A antibody
      • A1AT antibody
      • A1AT_HUMAN antibody
      • AAT antibody
      • Alpha 1 antiproteinase antibody
      • Alpha 1 antitrypsin antibody
      • Alpha 1 antitrypsin null antibody
      • Alpha 1 protease inhibitor antibody
      • Alpha-1 protease inhibitor antibody
      • Alpha-1-antiproteinase antibody
      • alpha1 proteinase inhibitor antibody
      • Alpha1AT antibody
      • Dom1 antibody
      • PI antibody
      • PI1 antibody
      • PRO0684 antibody
      • PRO2275 antibody
      • Serine (or cysteine) proteinase inhibitor clade A member 1 antibody
      • Serine protease inhibitor 1-1 antibody
      • Serine protease inhibitor A1a antibody
      • Serpin A1 antibody
      • Serpin A1a antibody
      • Serpin peptidase inhibitor clade A member 1 antibody
      • Serpina1 antibody
      • Short peptide from AAT antibody
      • SPAAT antibody
      • Spi1-1 antibody
      see all


    This product has been referenced in:

    • Meier F  et al. Hepatic differentiation of human iPSCs in different 3D models: A comparative study. Int J Mol Med 40:1759-1771 (2017). ELISA ; Human . Read more (PubMed: 29039463) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A


    Thank you for getting back to me. Unfrotunately we do not sell our sheep polyclonal ab8768 alpha 1 antitrypsin antibody in an unconjugated form. I am sorry I cannot be of more assistance. Would it be possible to use it in its conjugated form or would the HRP conjugation interfer with your assay?

    Read More
    Human Purified protein
    Blocking step
    Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0,1%


    Verified customer

    Submitted Dec 07 2005


    Thank you for your interest in our alpha 1 antitrypsin antibodies. I would recommend using ab7635 (goat antibody) as this antibody has been tested in ELISA, cross reacts with human, and is already HRP conjugated for your convenience. The antibody can be used at a dilution range of 1:4000 to 1:14000 and as the antibody concentration is 10mg/ml I have calculated this range is therefore 714ng/ml-2.5ug/ml. I hope this information will help you, please do not hesitate to contact me again if you need further help,

    Read More

    For licensing inquiries, please contact partnerships@abcam.com

    Sign up