Key features and details
- Rabbit polyclonal to alpha 1 Antitrypsin, prediluted
- Suitable for: IHC-P
- Reacts with: Horse, Human, Mink, Monkey, Baboon
- Isotype: IgG
Product nameAnti-alpha 1 Antitrypsin antibody, prediluted
See all alpha 1 Antitrypsin primary antibodies
DescriptionRabbit polyclonal to alpha 1 Antitrypsin, prediluted
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Horse, Human, Mink, Monkey, Baboon
Does not react with: Mouse, Sheep, Goat, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Kangaroo
Full length purified human serum alpha 1 Antitrypsin protein
- Human tonsil.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.60
Preservative: 0.097% Sodium azide
Constituent: 7.9% Tris HCl
Concentration information loading...
Our Abpromise guarantee covers the use of ab74664 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionInhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
Tissue specificityUbiquitous. Expressed in leukocytes and plasma.
Involvement in diseaseAlpha-1-antitrypsin deficiency
Sequence similaritiesBelongs to the serpin family.
DomainThe reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
modificationsN-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
Cellular localizationSecreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix.
- Information by UniProt
- A1A antibody
- A1AT antibody
- A1AT_HUMAN antibody
ab74664 has not yet been referenced specifically in any publications.