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  1. Link

    alpha-1-antitrypsin-antibody-prediluted-ab922.pdf

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Anti-alpha 1 Antitrypsin antibody, prediluted (ab922)

  • Datasheet
Submit a review Q&A (3)References (1)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody, prediluted (ab922)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Antitrypsin antibody, prediluted (ab922)

Key features and details

  • Rabbit polyclonal to alpha 1 Antitrypsin, prediluted
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Recombinant Human alpha 1 Antitrypsin protein (ab79181)

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Overview

  • Product name

    Anti-alpha 1 Antitrypsin antibody, prediluted
    See all alpha 1 Antitrypsin primary antibodies
  • Description

    Rabbit polyclonal to alpha 1 Antitrypsin, prediluted
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human alpha 1 Antitrypsin. Alpha-1-antitrypsin isolated from human serum.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.3
    Preservative: 0.05% Sodium azide

    Inert stabilizer
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Myeloma

    unknown
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Serum Proteins
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human alpha 1 Antitrypsin protein (ab79181)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
1/1.
Notes
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
1/1.

Target

  • Function

    Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
    Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
  • Tissue specificity

    Ubiquitous. Expressed in leukocytes and plasma.
  • Involvement in disease

    Alpha-1-antitrypsin deficiency
  • Sequence similarities

    Belongs to the serpin family.
  • Domain

    The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • Post-translational
    modifications

    N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
    Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
  • Cellular localization

    Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix.
  • Target information above from: UniProt accession P01009 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5265 Human
    • Omim: 107400 Human
    • SwissProt: P01009 Human
    • Unigene: 525557 Human
    • Alternative names

      • A1A antibody
      • A1AT antibody
      • A1AT_HUMAN antibody
      • AAT antibody
      • Alpha 1 antiproteinase antibody
      • Alpha 1 antitrypsin antibody
      • Alpha 1 antitrypsin null antibody
      • Alpha 1 protease inhibitor antibody
      • Alpha-1 protease inhibitor antibody
      • Alpha-1-antiproteinase antibody
      • alpha1 proteinase inhibitor antibody
      • Alpha1AT antibody
      • Dom1 antibody
      • PI antibody
      • PI1 antibody
      • PRO0684 antibody
      • PRO2275 antibody
      • Serine (or cysteine) proteinase inhibitor clade A member 1 antibody
      • Serine protease inhibitor 1-1 antibody
      • Serine protease inhibitor A1a antibody
      • Serpin A1 antibody
      • Serpin A1a antibody
      • Serpin peptidase inhibitor clade A member 1 antibody
      • Serpina1 antibody
      • Short peptide from AAT antibody
      • SPAAT antibody
      • Spi1-1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody, prediluted (ab922)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody, prediluted (ab922)
      Human normal placenta. Staining is observed as extracellular and cytoplasmic. Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for rabbit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
    • Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Antitrypsin antibody, prediluted (ab922)
      Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Antitrypsin antibody, prediluted (ab922)
      ICC/IF image of ab922 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab922, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab922? Please let us know so that we can cite the reference in this datasheet.

    ab922 has been referenced in 1 publication.

    • Perea-Resa C  et al. Cohesin Removal Reprograms Gene Expression upon Mitotic Entry. Mol Cell 78:127-140.e7 (2020). PubMed: 32035037

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Question

    I would like to know if your Anti-alpha 1 Antitrypsin antibody, prediluted (ab922) is specific as a hepatocyte marker. I would also want to know if there are publications you are aware of, citing the product.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 24 2013

    Answer



    All the information and references available for our products are contained on its datasheet. These references are updated as soon as any new information is brought to our attention. Unfortunately we don’t have any references for this antibody for the time being.

    This antibody is a mature hepatocyte marker. Alternatives specific markers for these cells are albumin, Fetoprotein, Annexin I and Annexin II (useful markers for detecting not only actively proliferating hepatocytes but also hepatocytes in preproliferative and postproliferative stages).

    Read More

    Ariana Veiga

    Abcam Scientific Support

    Answered on Jul 24 2013

    Question

    I have a question concerning your product:alpha 1-Antitrypsin antibody, product code ab922. In the information about specifity you wrote that it stains only macrophages and it does't stain any other cell types. Is it true? Mayby you can tell me what are specific markers of differentiated hepatocyte? I am going to work with human hepatocyte cell line. Do you have any antibodies against such markers?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 14 2004

    Answer

    This antibody reacts primarily with AAT because only one precipitating arch appears in immunoelectrophoresis. Contaminating antibodies were removed by solid phase absorption. I'm afraid that I am unable to advise which antibody would be suitable for use as a specific marker of differentiated hepatocytes.

    Read More

    Abcam Scientific Support

    Answered on Jan 21 2004

    Question

    1) With which other proteins have you tested the cross-reactivity of this antibody? May it be useful to target alpha1-antitrypsin from rats and hamsters? 2) Is there any chance that it may also work for ELISA?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 20 2002

    Answer

    This antibody is sourced externally, therefore all the information we have for this product is stated on the datasheet. The datasheet says "The antibody is specific for human alpha-1-antitrypsin.......stains macrophages in human tissues and does not stain any other cell type.....cross reactivity against monkey, dog and rat. Not tested in other species". Since this antibody is polyclonal and raised to whole A1AT, it is likely that it will work in an ELISA, but we can't guarantee this until tested.

    Read More

    Abcam Scientific Support

    Answered on Aug 21 2002

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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