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    alpha-1-antitrypsin-antibody-tmf1-4b5-ab116604.pdf

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Cardiovascular Blood Serum Proteins
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Anti-alpha 1 Antitrypsin antibody [TMF1 4B5] (ab116604)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (1)

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Key features and details

  • Mouse monoclonal [TMF1 4B5] to alpha 1 Antitrypsin
  • Reacts with: Mouse, Human
  • Isotype: IgG2a

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Primary
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HRP Anti-alpha 1 Antitrypsin antibody (ab191350)
Protein
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Recombinant Human alpha 1 Antitrypsin protein (ab79181)

View more associated products

Overview

  • Product name

    Anti-alpha 1 Antitrypsin antibody [TMF1 4B5]
    See all alpha 1 Antitrypsin primary antibodies
  • Description

    Mouse monoclonal [TMF1 4B5] to alpha 1 Antitrypsin
  • Host species

    Mouse
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Purified Human alpha 1 Antitrypsin.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99.85% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    TMF1 4B5
  • Isotype

    IgG2a
  • Research areas

    • Cardiovascular
    • Blood
    • Serum Proteins
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant Human alpha 1 Antitrypsin protein (ab79181)

Target

  • Function

    Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
    Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
  • Tissue specificity

    Ubiquitous. Expressed in leukocytes and plasma.
  • Involvement in disease

    Alpha-1-antitrypsin deficiency
  • Sequence similarities

    Belongs to the serpin family.
  • Domain

    The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • Post-translational
    modifications

    N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
    Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
  • Cellular localization

    Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix.
  • Target information above from: UniProt accession P01009 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5265 Human
    • Omim: 107400 Human
    • SwissProt: P01009 Human
    • Unigene: 525557 Human
    • Alternative names

      • A1A antibody
      • A1AT antibody
      • A1AT_HUMAN antibody
      • AAT antibody
      • Alpha 1 antiproteinase antibody
      • Alpha 1 antitrypsin antibody
      • Alpha 1 antitrypsin null antibody
      • Alpha 1 protease inhibitor antibody
      • Alpha-1 protease inhibitor antibody
      • Alpha-1-antiproteinase antibody
      • alpha1 proteinase inhibitor antibody
      • Alpha1AT antibody
      • Dom1 antibody
      • PI antibody
      • PI1 antibody
      • PRO0684 antibody
      • PRO2275 antibody
      • Serine (or cysteine) proteinase inhibitor clade A member 1 antibody
      • Serine protease inhibitor 1-1 antibody
      • Serine protease inhibitor A1a antibody
      • Serpin A1 antibody
      • Serpin A1a antibody
      • Serpin peptidase inhibitor clade A member 1 antibody
      • Serpina1 antibody
      • Short peptide from AAT antibody
      • SPAAT antibody
      • Spi1-1 antibody
      see all

    Protocols

    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab116604? Please let us know so that we can cite the reference in this datasheet.

    ab116604 has been referenced in 1 publication.

    • Yin H  et al. Quantitative Analysis of a-1-Antitrypsin Glycosylation Isoforms in HCC Patients Using LC-HCD-PRM-MS. Anal Chem 92:8201-8208 (2020). PubMed: 32426967

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Customer kindly contacted us regarding the molecular weight of ab116604, ab129194, ab769.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 17 2012

    Answer

    Thank you for contacting Abcam.

    Since all three antibodies have IgG (or a IgG subclass) isotypes, the molecular weight of each is approximately 160kDa (23-25kDa for both light chains, and 53kDa for both heavy chains).

    Read More

    Abcam Scientific Support

    Answered on Dec 17 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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