The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
1/1000. Predicted molecular weight: 100 kDa.
Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.
Expressed ubiquitously in normal tissues.
Belongs to the vinculin/alpha-catenin family.
Cell membrane and Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse brain labeling alpha 1 Catenin with ab189310 at 5 µg/ml followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MCF-7 cells (bottom histogram) labeling alpha 1 Catenin with ab189310 at 1/10 dilution and a negative control (top). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot - Anti-alpha 1 Catenin antibody - N-terminal (ab189310)
Anti-alpha 1 Catenin antibody - N-terminal (ab189310) at 1/1000 dilution + mouse bladder tissue lysate at 35 µg