Key features and details
- Mouse monoclonal  to alpha 1 Fetoprotein (APC)
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG1
Product nameAnti-alpha 1 Fetoprotein antibody  (APC)
See all alpha 1 Fetoprotein primary antibodies
DescriptionMouse monoclonal  to alpha 1 Fetoprotein (APC)
ConjugationAPC. Ex: 645nm, Em: 660nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Recombinant full length protein (His-tag) corresponding to Human alpha 1 Fetoprotein aa 1-609 (C terminal).
Database link: P02771
- Flow Cyt: HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab275573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
FunctionBinds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Tissue specificityPlasma. Synthesized by the fetal liver and yolk sac.
Sequence similaritiesBelongs to the ALB/AFP/VDB family.
Contains 3 albumin domains.
Developmental stageOccurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
modificationsIndependent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
- Information by UniProt
- Afp antibody
- AFPD antibody
- Alpha fetoglobulin antibody
Flow cytometric analysis of HepG2 (human liver hepatocellular carcinoma cell line) cells labeling alpha 1 Fetoprotein with ab275573 (Black) compared with isotype control (Grey). The cells were treated according to manufacturers manual. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab275573 has not yet been referenced specifically in any publications.