Overview

  • Product name
    Anti-alpha 1 Fetoprotein antibody [4A3]
    See all alpha 1 Fetoprotein primary antibodies
  • Description
    Mouse monoclonal [4A3] to alpha 1 Fetoprotein
  • Host species
    Mouse
  • Specificity
    ab10071 and 10072 react with different determinants of human a-fetoprotein. They do not react with albumin.
  • Tested applications
    Suitable for: Sandwich ELISA, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (purified) (Human).

  • General notes
    Concentration varies from lot to lot and can be provided on request.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

Properties

Applications

Our Abpromise guarantee covers the use of ab10071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [5H7] to alpha 1 Fetoprotein (HRP) (ab10072). Can be used as capture antibody.
ELISA Use at an assay dependent dilution.

Target

  • Function
    Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
  • Tissue specificity
    Plasma. Synthesized by the fetal liver and yolk sac.
  • Sequence similarities
    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Developmental stage
    Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
  • Post-translational
    modifications
    Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
    Sulfated.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Afp antibody
    • AFPD antibody
    • Alpha fetoglobulin antibody
    • Alpha fetoprotein antibody
    • Alpha fetoprotein precursor antibody
    • Alpha-1-fetoprotein antibody
    • Alpha-fetoglobulin antibody
    • Alpha-fetoprotein antibody
    • alpha-fetoprotein, Hereditary persistence of, included antibody
    • FETA antibody
    • FETA_HUMAN antibody
    • Hereditary persistence of alpha fetoprotein antibody
    • HPAFP antibody
    see all

References

This product has been referenced in:
  • Selden C  et al. A clinical-scale BioArtificial Liver, developed for GMP, improved clinical parameters of liver function in porcine liver failure. Sci Rep 7:14518 (2017). Read more (PubMed: 29109530) »
  • Kilbride P  et al. Spatial considerations during cryopreservation of a large volume sample. Cryobiology 73:47-54 (2016). ELISA ; Human . Read more (PubMed: 27256662) »
See all 5 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thanks for your reply, and I am sorry that I don't have a better explanation for this problem. If there is anything else that we can do for you, please let me know and I'll be happy to help.

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Answer

I just wanted to thank you for completing our follow-up survey recently, and I'm sorry to see that this problem persisted even with the replacement vial of antibody. Since the results are still not satisfactory, I would be happy to issue a credit or refund for your purchase, or I can send a new vial of your choosing. Please let me know which you would prefer, or if there is anything else that we can do for you.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Cell (Liver cell culture)
Specification
Liver cell culture
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Type
Sandwich (Capture)

Dr. Eva Schmelzer

Verified customer

Submitted Jul 20 2010

Answer

Thank you for your enquiry. Unless a dilution is listed on the data sheet, we do not know the dilution to use for sandwich ELISA. A few things to keep in mind are that typically you do not need a lot of antibody to do an ELISA, just enough to coat the wells. Perhaps you could start with 0.1 ug/mL. ELISAs can be tricky because there is a bit of trial and error involved if you don't have a recommended dilution. It may take some time to optimize your assay. Maybe you could try a few dilutions between 0.1 ug/ml and 5 ug/ml and see how that goes. I am sorry that I do not have more information. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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