Product nameAnti-alpha 1 Fetoprotein antibody
See all alpha 1 Fetoprotein primary antibodies
DescriptionRabbit polyclonal to alpha 1 Fetoprotein
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human alpha 1 Fetoprotein aa 260-609. The exact sequence is proprietary. BC027881
L DVAHVHEHCC RGDVLDCLQD GEKIMSYICS QQDTLSNKIT ECCKLTTLER GQCIIHAEND EKPEGLSPNL NRFLGDRDFN QFSSGEKNIF LASFVHEYSR RHPQLAVSVI LRVAKGYQEL LEKCFQTENP LECQDKGEEE LQKYIQESQA LAKRSCGLFQ KLGEYYLQNA FLVAYTKKAP QLTSSELMAI TRKMAATAAT CCQLSEDKLL ACGEGAADII IGHLCIRHEM TPVNPGVGQC CTSSYANRRP CFSSLVVDET YVPPAFSDDK FIFHKDLCQA QGVALQTMKQ EFLINLVKQK PQITEEQLEA VIADFSGLLE KCCQGQEQEV CFAEEGQKLI SKTRAALGV
Database link: P02771
- Human liver cancer tissue
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab182645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 69 kDa.|
FunctionBinds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Tissue specificityPlasma. Synthesized by the fetal liver and yolk sac.
Sequence similaritiesBelongs to the ALB/AFP/VDB family.
Contains 3 albumin domains.
Developmental stageOccurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
modificationsIndependent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
- Information by UniProt
- Afp antibody
- AFPD antibody
- Alpha fetoglobulin antibody
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling Alpha 1 fetoprotein with ab182645 at 1/60 dilution (left). On the right is treated with the immunogen.
Anti-alpha 1 Fetoprotein antibody (ab182645) at 1/1000 dilution + Human liver cancer tissue at 40 µg
Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
Predicted band size: 69 kDa
Exposure time: 30 seconds
ab182645 has been referenced in 1 publication.
- Liu W et al. Hepatic epithelioid angiomyolipoma is a rare and potentially severe but treatable tumor: A report of three cases and review of the literature. Oncol Lett 11:3669-3675 (2016). IHC ; Human . PubMed: 27313680