Overview

  • Product name

    Anti-alpha 1 Fetoprotein antibody
    See all alpha 1 Fetoprotein primary antibodies
  • Description

    Rabbit polyclonal to alpha 1 Fetoprotein
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Horse, Cow, Dog, Pig, Chimpanzee, Gorilla
  • Immunogen

    Recombinant fragment corresponding to Human alpha 1 Fetoprotein aa 378-609 (C terminal). E.coli-derived.
    Sequence:

    QEL LEKCFQTENP LECQDKGEEE LQKYIQESQA LAKRSCGLFQ KLGEYYLQNA FLVAYTKKAP QLTSSELMAI TRKMAATAAT CCQLSEDKLL ACGEGAADII IGHLCIRHEM TPVNPGVGQC CTSSYANRRP CFSSLVVDET YVPPAFSDDK FIFHKDLCQA QGVALQTMKQ EFLINLVKQK PQITEEQLEA VIADFSGLLE KCCQGQEQEV CFAEEGQKLI SKTRAALGV


    Database link: P02771

  • Positive control

    • WB: Recombinant human alpha 1 Fetoprotein protein; HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab217325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 69 kDa.

The detection limit for AFP is approximately 0.25 ng/lane under reducing conditions.

Target

  • Function

    Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
  • Tissue specificity

    Plasma. Synthesized by the fetal liver and yolk sac.
  • Sequence similarities

    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Developmental stage

    Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
  • Post-translational
    modifications

    Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
    Sulfated.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Afp antibody
    • AFPD antibody
    • Alpha fetoglobulin antibody
    • Alpha fetoprotein antibody
    • Alpha fetoprotein precursor antibody
    • Alpha-1-fetoprotein antibody
    • Alpha-fetoglobulin antibody
    • Alpha-fetoprotein antibody
    • alpha-fetoprotein, Hereditary persistence of, included antibody
    • FETA antibody
    • FETA_HUMAN antibody
    • Hereditary persistence of alpha fetoprotein antibody
    • HPAFP antibody
    see all

Images

  • Anti-alpha 1 Fetoprotein antibody (ab217325) at 0.5 µg/ml + HepG2 whole cell lysate at 40 µg

    Predicted band size: 69 kDa

  • Anti-alpha 1 Fetoprotein antibody (ab217325) at 0.5 µg/ml + human recombinant AFP protein, 0.5 ng

    Predicted band size: 69 kDa

References

ab217325 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab217325.
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