Overview

  • Product name

    Anti-alpha 1 Fetoprotein antibody
    See all alpha 1 Fetoprotein primary antibodies
  • Description

    Rabbit polyclonal to alpha 1 Fetoprotein
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Full length native protein (purified) within Human alpha 1 Fetoprotein aa 1 to the C-terminus. The exact sequence is proprietary. (Purified from human cord serum).
    Database link: P02771

  • Positive control

    • IHC-P: Human fetal liver tissue. WB: HepG2 whole cell lysate (ab7900).

Properties

Applications

Our Abpromise guarantee covers the use of ab227800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200.

Primary antibody incubation for 10 minutes at room temperature.

WB 1/25. Predicted molecular weight: 68 kDa.

Primary antibody incubation for 1 hour at room temperature.

Target

  • Function

    Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
  • Tissue specificity

    Plasma. Synthesized by the fetal liver and yolk sac.
  • Sequence similarities

    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Developmental stage

    Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
  • Post-translational
    modifications

    Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
    Sulfated.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Afp antibody
    • AFPD antibody
    • Alpha fetoglobulin antibody
    • Alpha fetoprotein antibody
    • Alpha fetoprotein precursor antibody
    • Alpha-1-fetoprotein antibody
    • Alpha-fetoglobulin antibody
    • Alpha-fetoprotein antibody
    • alpha-fetoprotein, Hereditary persistence of, included antibody
    • FETA antibody
    • FETA_HUMAN antibody
    • Hereditary persistence of alpha fetoprotein antibody
    • HPAFP antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human fetal liver tissue stained for alpha 1 Fetoprotein using ab227800 at 1/200 dilution in immunohistochemical analysis.

  • Anti-alpha 1 Fetoprotein antibody (ab227800) at 1/25 dilution + HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

    Predicted band size: 68 kDa

References

ab227800 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227800.
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