Product nameAnti-alpha 1 Fetoprotein antibody
See all alpha 1 Fetoprotein primary antibodies
DescriptionRabbit polyclonal to alpha 1 Fetoprotein
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivityReacts with: Mouse, Rat, Cat, Human
A synthetic peptide corresponding to residues in C-terminus of human AFP.
- HepG2 cell lysate; Human liver tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab46799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 69 kDa).|
|IHC-P||1/100 - 1/250.|
FunctionBinds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Tissue specificityPlasma. Synthesized by the fetal liver and yolk sac.
Sequence similaritiesBelongs to the ALB/AFP/VDB family.
Contains 3 albumin domains.
Developmental stageOccurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
modificationsIndependent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
- Information by UniProt
- Afp antibody
- AFPD antibody
- Alpha fetoglobulin antibody
ab46799 staining alpha 1 Fetoprotein in cat liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2.5% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in 10mM citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/50 in PBS + 1X casein) for 1 hour at 25°C. An undiluted HRP-conjugated horse anti-rabbit IgG polyclonal was used as the secondary antibody.
All lanes : Anti-alpha 1 Fetoprotein antibody (ab46799) at 1 µg/ml
Lane 1 : HepG2 (Human) Whole Cell Lysate
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted?
Additional bands at: 58 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab46799 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Ab46799 staining alpha 1 Fetoprotein in Mouse tumour tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue samples were fixed by paraformaldehyde, permeabilized with 0.3% triton X-100, blocked with 5% FBS for 1 hour at 22°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/100 in antibody diluent) at 4°C for 12 hours. A HRP conjugated goat polyclonal anti-rabbit IgG (1/250) was used as the secondary antibody.
Anti-alpha 1 Fetoprotein antibody (ab46799) at 1/50000 dilution + HepG2 cell lysate at 10 µg
Goat anti-rabbit HRP labeled, at 1/2000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDa
ab46799 staining alpha 1 Fetoprotein in Mouse embryonic liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formalin and blocked with 2.5% serum for 20 minutes at 23°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/750 in 1% BSA in TBS) for 1 hour at 23°C. An undiluted HRP-conjugated Horse anti-rabbit polyclonal was used as the secondary antibody.
This product has been referenced in:
- Yu Y et al. MiR-490-5p inhibits the stemness of hepatocellular carcinoma cells by targeting ECT2. J Cell Biochem 120:967-976 (2019). Read more (PubMed: 30206962) »
- Chen J et al. Deletion of sphingosine kinase 1 inhibits liver tumorigenesis in diethylnitrosamine-treated mice. Oncotarget 9:15635-15649 (2018). Read more (PubMed: 29643998) »