Anti-alpha 1 Fetoprotein antibody (ab8201)


  • Product name
    Anti-alpha 1 Fetoprotein antibody
    See all alpha 1 Fetoprotein primary antibodies
  • Description
    Rabbit polyclonal to alpha 1 Fetoprotein
  • Host species
  • Specificity
    Specificity of the antisera is confirmed by ELISA (OPD) on human alpha-Fetoprotein versus albumin, IgG, IgM. Ouchterlony double diffusion, immunoelectrophoresis. No crossreactivity with human plasma proteins.
  • Tested applications
    Suitable for: Sandwich ELISA, Othermore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human).



Our Abpromise guarantee covers the use of ab8201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 0.1 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal to alpha 1 Fetoprotein (ab54745).

For sandwich ELISA, use this antibody as Detection at 0.1µg/ml with ab54745 as Capture.

AP Use at an assay dependent concentration.
Other Use at an assay dependent concentration.


  • Function
    Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
  • Tissue specificity
    Plasma. Synthesized by the fetal liver and yolk sac.
  • Sequence similarities
    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Developmental stage
    Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
  • Post-translational
    Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Afp antibody
    • AFPD antibody
    • Alpha fetoglobulin antibody
    • Alpha fetoprotein antibody
    • Alpha fetoprotein precursor antibody
    • Alpha-1-fetoprotein antibody
    • Alpha-fetoglobulin antibody
    • Alpha-fetoprotein antibody
    • alpha-fetoprotein, Hereditary persistence of, included antibody
    • FETA antibody
    • FETA_HUMAN antibody
    • Hereditary persistence of alpha fetoprotein antibody
    • HPAFP antibody
    see all




ab8201 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us.
These are very similar products, both purified polycloanl antibodies to alpha 1 fetoprotein. The main differences are the concentration of the antibody (ab8201 is supplied at 1 mg/ml and ab19529 is at 3.2 mg/ml) and th...

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Thank you for your enquiry regarding ab8201. I can confirm that the concentration after reconstitution is 1 mg/ml. I hope this helps and if I can assist further, please do not hesitate to contact me.

That's good! And thank you for your feedback.

You purchased ab8201 via an online order (reference 23563)on 11 September and paid by VISA. Our accounts department will credit your VISA card for the cost of this antibody (USD 293.00). For security reasons, we no longer have your card number details ...

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I have continued to chase our supplier over the Christmas period for more data about how this antibody has been used in WB on fetal and adult sera. In particular I had requested a protocol on how they prepared the fetal sera. After much chasing I now f...

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At last I have some further information for you regarding the use of ab8201 in WB. The following information comes from the researcher who tested the antibody: "WB from SDS-PAGE (10%, reduced) was made with AFP load 0.5-2.0 ug/band transferred to nitro...

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Your comments are interesting - thank you for your honesty. I will address each of the issues you raise below but if you wish to discuss this matter further, please telephone me on +44 1223 472030. Currently, we have in excess of 6,000 products in ...

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All the information we have for ab8201 -- Rabbit polyclonal to human Alpha-1 Fetoprotein -- is on the datasheet. I am afraid that we do not have any information about whether this antibody cross reacts with rat so I am unable to advise how you may twea...

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The ELISA protocol on our website is a general protocol for antigen assay. For further information about different ELISA protocols, I suggest that you look in Harlow & Lane, Using Antibodies - A laboratory Manual, Cold Spring Harbor Laboratory Press.

This antibody has never been tested for cross reactivity in mouse, therefore we can't guarantee results. I've looked at the amino acid sequences for both mouse and human AFP and they appear to be quite different. In light of this, even though this a...

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