Key features and details
- Mouse monoclonal [C2] to alpha 1 Fetoprotein - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG1
Product nameAnti-alpha 1 Fetoprotein antibody [C2] - BSA and Azide free
See all alpha 1 Fetoprotein primary antibodies
DescriptionMouse monoclonal [C2] to alpha 1 Fetoprotein - BSA and Azide free
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, GorillaDoes not react with: Rat, Cow, Dog
Full length native protein (purified) corresponding to Human alpha 1 Fetoprotein aa 19-609. purified from serum of a hepatoma patient.
RTLHRNEYGIASILDSYQCTAEISLADLATIFFAQFVQEATYKEVSKMVK DALTAIEKPTGDEQSSGCLENQLPAFLEELCHEKEILEKYGHSDCCSQSE EGRHNCFLAHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMNKFIYEIAR RHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSL LNQHACAVMKNFGTRTFQAITVTKLSQKFTKVNFTEIQKLVLDVAHVHEH CCRGDVLDCLQDGEKIMSYICSQQDTLSNKITECCKLTTLERGQCIIHAE NDEKPEGLSPNLNRFLGDRDFNQFSSGEKNIFLASFVHEYSRRHPQLAVS VILRVAKGYQELLEKCFQTENPLECQDKGEEELQKYIQESQALAKRSCGL FQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAATAATCCQLSEDK LLACGEGAADIIIGHLCIRHEMTPVNPGVGQCCTSSYANRRPCFSSLVVD ETYVPPAFSDDKFIFHKDLCQAQGVALQTMKQEFLINLVK QKPQITEE QLEAVIADFSGLLEKCCQGQEQEVCFAEEGQKLISKTRAALGV
Database link: P02771
- HepG2 cells; Human fetal liver or hepatocellular carcinoma tissues.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: 100% PBS
Concentration information loading...
PurityProtein A/G purified
Purification notesab212325 is purified from Bioreactor Concentrate by Protein A/G.
Light chain typekappa
Our Abpromise guarantee covers the use of ab212325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
for 30 minutes at RT
FunctionBinds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Tissue specificityPlasma. Synthesized by the fetal liver and yolk sac.
Sequence similaritiesBelongs to the ALB/AFP/VDB family.
Contains 3 albumin domains.
Developmental stageOccurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
modificationsIndependent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
- Information by UniProt
- Afp antibody
- AFPD antibody
- Alpha fetoglobulin antibody
ab212325 has not yet been referenced specifically in any publications.