Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1017Y] to alpha 1 Fetoprotein
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Product nameAnti-alpha 1 Fetoprotein antibody [EP1017Y]
See all alpha 1 Fetoprotein primary antibodies
DescriptionRabbit monoclonal [EP1017Y] to alpha 1 Fetoprotein
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt or ICC/IF
Species reactivityReacts with: Human
Synthetic peptide within Human alpha 1 Fetoprotein (C terminal). The exact sequence is proprietary.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab45147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Predicted molecular weight: 69 kDa.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionBinds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Tissue specificityPlasma. Synthesized by the fetal liver and yolk sac.
Sequence similaritiesBelongs to the ALB/AFP/VDB family.
Contains 3 albumin domains.
Developmental stageOccurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
modificationsIndependent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
- Information by UniProt
- Afp antibody
- AFPD antibody
- Alpha fetoglobulin antibody
Anti-alpha 1 Fetoprotein antibody [EP1017Y] (ab45147) at 1/50000 dilution + HepG2 membrane
Predicted band size: 69 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Ab45147 staining human alpha 1 Fetoprotein in human fetal liver by immunohistochemistry using paraffin embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab45147 has been referenced in 1 publication.
- Joshi M et al. Chemokine-mediated robust augmentation of liver engraftment: a novel approach. Stem Cells Transl Med 4:21-30 (2015). PubMed: 25473087