Recombinant
RabMAb

Recombinant Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)

Overview

  • Product name

    Anti-alpha 1 Fetoprotein antibody [EPAFP61]
    See all alpha 1 Fetoprotein primary antibodies
  • Description

    Rabbit monoclonal [EPAFP61] to alpha 1 Fetoprotein
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human alpha 1 Fetoprotein. The exact sequence is proprietary.

  • Positive control

    • HepG2 and Human fetal liver lysates; Human hepatocellular carcinoma tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat up to 98°C, below boiling, and then let cool for 10-20 min.

ICC 1/100 - 1/250.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
  • Tissue specificity

    Plasma. Synthesized by the fetal liver and yolk sac.
  • Sequence similarities

    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Developmental stage

    Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
  • Post-translational
    modifications

    Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
    Sulfated.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Afp antibody
    • AFPD antibody
    • Alpha fetoglobulin antibody
    • Alpha fetoprotein antibody
    • Alpha fetoprotein precursor antibody
    • Alpha-1-fetoprotein antibody
    • Alpha-fetoglobulin antibody
    • Alpha-fetoprotein antibody
    • alpha-fetoprotein, Hereditary persistence of, included antibody
    • FETA antibody
    • FETA_HUMAN antibody
    • Hereditary persistence of alpha fetoprotein antibody
    • HPAFP antibody
    see all

Images

  • All lanes : Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Human fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 68 kDa

  • Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma labelling alpha 1 Fetoprotein with ab133617 at 1/100 dilution.
  • ab133617 showing negative staining in Breast carcinoma tissue.

  • ab133617 showing negative staining in Glioma tissue.

  • ab133617 showing positive staining in Hepatocellular carcinoma poor differentiated tissue.

  • ab133617 showing positive staining in Hepatocellular carcinoma well differentiated tissue.

  • ab133617 showing negative staining in Normal liver tissue.

  • ab133617 showing negative staining in Lung adenocarcinoma tissue.

  • ab133617 showing negative staining in Normal tonsil tissue.

  • ab133617 showing negative staining in Colonic adenocarcinoma tissue.

References

This product has been referenced in:

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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