Recombinant
RabMAb

Recombinant Anti-alpha 1 Fetoprotein antibody [EPAFP61] - Low endotoxin, Azide free (ab215379)

Overview

  • Product name

    Anti-alpha 1 Fetoprotein antibody [EPAFP61] - Low endotoxin, Azide free
    See all alpha 1 Fetoprotein primary antibodies
  • Description

    Rabbit monoclonal [EPAFP61] to alpha 1 Fetoprotein - Low endotoxin, Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length Human alpha 1 Fetoprotein protein.

  • Positive control

    • HepG2 and Human fetal liver lysates; Human hepatocellular carcinoma tissue.
  • General notes

    ab215379 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab215379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.

ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
  • Tissue specificity

    Plasma. Synthesized by the fetal liver and yolk sac.
  • Sequence similarities

    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Developmental stage

    Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
  • Post-translational
    modifications

    Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
    Sulfated.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Afp antibody
    • AFPD antibody
    • Alpha fetoglobulin antibody
    • Alpha fetoprotein antibody
    • Alpha fetoprotein precursor antibody
    • Alpha-1-fetoprotein antibody
    • Alpha-fetoglobulin antibody
    • Alpha-fetoprotein antibody
    • alpha-fetoprotein, Hereditary persistence of, included antibody
    • FETA antibody
    • FETA_HUMAN antibody
    • Hereditary persistence of alpha fetoprotein antibody
    • HPAFP antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma labelling alpha 1 Fetoprotein with ab133617 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133617).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab133617 showing negative staining in Breast carcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133617).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab133617 showing negative staining in Glioma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133617).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab133617 showing positive staining in Hepatocellular carcinoma poor differentiated tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133617).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab133617 showing positive staining in Hepatocellular carcinoma well differentiated tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133617).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab133617 showing negative staining in Normal liver tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133617).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab133617 showing negative staining in Lung adenocarcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133617).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab133617 showing negative staining in Normal tonsil tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133617).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab133617 showing negative staining in Colonic adenocarcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133617).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

References

ab215379 has not yet been referenced specifically in any publications.

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