RecombinantRabMAb

Recombinant Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)

Submit a review Submit a question References (3)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)
  • Western blot - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)
  • Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)
  • Flow Cytometry - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)
  • Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9309] to alpha 1 Fetoprotein
  • Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-alpha 1 Fetoprotein antibody [EPR9309]
    See all alpha 1 Fetoprotein primary antibodies

  • Description

    Rabbit monoclonal [EPR9309] to alpha 1 Fetoprotein

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human alpha 1 Fetoprotein. The exact sequence is proprietary.

  • Positive control

    • Human plasma, HepG2 and Human fetal liver lysates, Human moderately differentiated hepatocellular carcinoma and Human poorly differentiated hepatocellular carcinoma tissues, HepG2 cells

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab169552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
IP
Human
WB
Human
Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.
Flow Cyt
1/50.

ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.
Flow Cyt
1/50.

ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

  • Tissue specificity

    Plasma. Synthesized by the fetal liver and yolk sac.

  • Sequence similarities

    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.

  • Developmental stage

    Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.

  • Post-translational
    modifications

    Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
    Sulfated.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • Afp antibody
      • AFPD antibody
      • Alpha fetoglobulin antibody
      • Alpha fetoprotein antibody
      • Alpha fetoprotein precursor antibody
      • Alpha-1-fetoprotein antibody
      • Alpha-fetoglobulin antibody
      • Alpha-fetoprotein antibody
      • alpha-fetoprotein, Hereditary persistence of, included antibody
      • FETA antibody
      • FETA_HUMAN antibody
      • Hereditary persistence of alpha fetoprotein antibody
      • HPAFP antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)

      Immunohistochemical analysis of paraffin embedded human moderately differentiated hepatocellular carcinoma tissue labeling alpha 1 Fetoprotein with ab169552 antibody at 1/50.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)
      Western blot - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)
      All lanes : Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552) at 1/1000 dilution

      Lane 1 : Human plasma lysate
      Lane 2 : HepG2 lysate
      Lane 3 : Human fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 68 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)
      Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)
      Immunofluorescent analysis of HepG2 cells labeling alpha 1 Fetoprotein with ab169552 at 1/100.
    • Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)
      Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)
      Immunoprecipitation. ab169552 at 1/1000 staining alpha 1 Fetoprotein in HepG2 cell lysate immunoprecipitated using ab169552 at 1/10. Predicted band size : 68 kDa.
    • Flow Cytometry - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)
      Flow Cytometry - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)

      Flow Cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling alpha 1 Fetoprotein with purified ab169552 at 1/50 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)

      Immunohistochemical analysis of paraffin embedded Human poorly differentiated hepatocellular carcinoma tissue labeling alpha 1 Fetoprotein with ab169552 antibody at 1/50.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)
      Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)

    References (3)

    Publishing research using ab169552? Please let us know so that we can cite the reference in this datasheet.

    ab169552 has been referenced in 3 publications.

    • Song Y  et al. Oxytetracycline have the therapeutic efficiency in CD133+ HCC population through suppression CD133 expression by decreasing of protein stability of CD133. Sci Rep 8:16100 (2018). PubMed: 30382122
    • Yanagi Y  et al. In vivo and ex vivo methods of growing a liver bud through tissue connection. Sci Rep 7:14085 (2017). IHC ; Rat . PubMed: 29074999
    • Xin H  et al. Establishment and characterization of 7 novel hepatocellular carcinoma cell lines from patient-derived tumor xenografts. PLoS One 9:e85308 (2014). IHC-P ; Human . PubMed: 24416385

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