Anti-alpha 1 Fetoprotein antibody (FITC) (ab87636)

Overview

  • Product name

    Anti-alpha 1 Fetoprotein antibody (FITC)
    See all alpha 1 Fetoprotein primary antibodies
  • Description

    Rabbit polyclonal to alpha 1 Fetoprotein (FITC)
  • Host species

    Rabbit
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Specificity

    ab87636 does not cross react to human lactoferrin, human serum albumin, bovine lactoferrin or bovine serum albumin and other cellular proteins.
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human alpha 1 Fetoprotein. Purified human alpha Fetoprotein.

Properties

Applications

Our Abpromise guarantee covers the use of ab87636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 69 kDa.
ELISA 1/10000.

Target

  • Function

    Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
  • Tissue specificity

    Plasma. Synthesized by the fetal liver and yolk sac.
  • Sequence similarities

    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Developmental stage

    Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
  • Post-translational
    modifications

    Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
    Sulfated.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Afp antibody
    • AFPD antibody
    • Alpha fetoglobulin antibody
    • Alpha fetoprotein antibody
    • Alpha fetoprotein precursor antibody
    • Alpha-1-fetoprotein antibody
    • Alpha-fetoglobulin antibody
    • Alpha-fetoprotein antibody
    • alpha-fetoprotein, Hereditary persistence of, included antibody
    • FETA antibody
    • FETA_HUMAN antibody
    • Hereditary persistence of alpha fetoprotein antibody
    • HPAFP antibody
    see all

References

ab87636 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your inquiry.

I contacted the specialists in the lab and they provide the following information:

1.) The ratio of FITC to Ig is between 10:1 and 20:1.

2.) There is no modification on IgG to create additional amino groups. We used existing primary amino groups on IgG to make a covalent thiourea bond between activated FITC and the primary amino group present on IgG molecule. We protect the Fab amino groups by favoring the amino groups on Fc region to cross react with active FITC.

Charged groups in the antibody were not measured and will also depend on the buffer that is used.

3.) Since there is no spacer used between FITC and IgG the distance between IgG and FITC is the thiourea bond. So the size of the conjugate will be the size of the antibody plus the FITC molecule without an extra spacer.

I hope this information is helpful and wish you good luck with your research.

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