Key features and details
- Rabbit polyclonal to alpha 1 Glycine Receptor/GLRA1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-alpha 1 Glycine Receptor/GLRA1 antibody
See all alpha 1 Glycine Receptor/GLRA1 primary antibodies
DescriptionRabbit polyclonal to alpha 1 Glycine Receptor/GLRA1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Cow, Zebrafish, Rhesus monkey
Recombinant fragment within Human alpha 1 Glycine Receptor/GLRA1 (internal sequence). The exact sequence is proprietary.
Database link: P23415
- WB: HeLa S3 whole cell lysate. IHC-P: OVCA xenograft tissue. ICC/IF: DIV9 rat E18 primary cortical neuron cells.
This product was previously labelled as alpha 1 Glycine Receptor
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228541 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 53 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionThe glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing).
Involvement in diseaseDefects in GLRA1 are a cause of startle disease (STHE) [MIM:149400]; also known as hereditary hyperekplexia or congenital stiff-person syndrome. STHE is a genetically heterogeneous neurologic disorder characterized by muscular rigidity of central nervous system origin, particularly in the neonatal period, and by an exaggerated startle response to unexpected acoustic or tactile stimuli. Inheritance can be autosomal dominant or recessive.
Sequence similaritiesBelongs to the ligand-gated ion channel (TC 1.A.9) family. Glycine receptor (TC 1.A.9.3) subfamily. GLRA1 sub-subfamily.
Cellular localizationCell junction > synapse > postsynaptic cell membrane. Cell membrane.
- Information by UniProt
- GLRA1 antibody
- GLRA1_HUMAN antibody
- Glycine receptor 48 kDa subunit antibody
Paraffin-embedded OVCA (human) xenograft tissue stained for alpha 1 Glycine Receptor/GLRA1 using ab228541 at 1/100 dilution in immunohistochemical analysis.
4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neuron cells labeling alpha 1 Glycine Receptor/GLRA1 using ab228541 at 1/500 dilution.
The nuclear counterstain is DAPI (blue). beta Tubulin is labeled with an anti-beta Tubulin antibody (red).
Anti-alpha 1 Glycine Receptor/GLRA1 antibody (ab228541) at 1/1500 dilution + HeLa S3 whole cell lysate at 30 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 53 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228541 has not yet been referenced specifically in any publications.