Recombinant
RabMAb

Recombinant Anti-Alpha 1 microglobulin antibody [EPR5880] (ab129059)

Overview

  • Product name

    Anti-Alpha 1 microglobulin antibody [EPR5880]
    See all Alpha 1 microglobulin primary antibodies
  • Description

    Rabbit monoclonal [EPR5880] to Alpha 1 microglobulin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Alpha 1 microglobulin aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Human plasma lysate; Human liver tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 31 kDa (predicted molecular weight: 39 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.
      Trypstatin is a trypsin inhibitor.
    • Tissue specificity

      Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine.
    • Sequence similarities

      In the N-terminal section; belongs to the calycin superfamily. Lipocalin family.
      Contains 2 BPTI/Kunitz inhibitor domains.
    • Post-translational
      modifications

      The precursor is proteolytically processed into separately functioning proteins.
      3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-53, Lys-111, Lys-137, and Lys-149 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic; the human protein forms chromophore even when expressed in insect and bacterial cells. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region 'Cys-352'.
      Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate.
      Addition of glycosaminoglycan chondroitin sulfate, allows cross-linking between the different components.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • A1M antibody
      • Alpha 1 microglobulin/bikunin precursor antibody
      • Alpha 1 microglycoprotein antibody
      • Alpha-1 microglycoprotein antibody
      • Alpha-1-microglobulin antibody
      • AMBP antibody
      • AMBP_HUMAN antibody
      • Bikunin antibody
      • Complex-forming glycoprotein heterogeneous in charge antibody
      • EDC1 antibody
      • Growth inhibiting protein 19 antibody
      • HCP antibody
      • HI-30 antibody
      • HI30 antibody
      • IATIL antibody
      • ITI antibody
      • ITI-LC antibody
      • ITIL antibody
      • Microglobulin (alpha 1) antibody
      • Protein AMBP antibody
      • Protein HC antibody
      • Trypstatin antibody
      • Uristatin antibody
      • Uronic-acid-rich protein antibody
      • UTI antibody
      see all

    Images

    • ab129059 at 1/50 dilution staining Bikunin + Trypstatin in paraffin-embedded Human liver tissue by Immunohistochemistry.
    • Anti-Alpha 1 microglobulin antibody [EPR5880] (ab129059) at 1/1000 dilution + Human plasma lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 39 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab129059 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab129059.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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