Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)
![Immunocytochemistry - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)](https://www.abcam.com/ps/products/7/ab7671/Images/ab7671-18-anti-alpha-1-sodium-potassium-atpase-4646-immunocytochemistry-antibody-hek293-human.jpg "Immunocytochemistry - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)")
Key features and details
- Mouse monoclonal [464.6] to alpha 1 Sodium Potassium ATPase
- Suitable for: IHC-P, WB, ICC
- Reacts with: Mouse, Rat, Rabbit, Human, Pig
- Isotype: IgG1
Overview
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Product name
Anti-alpha 1 Sodium Potassium ATPase antibody [464.6]
See all alpha 1 Sodium Potassium ATPase primary antibodies -
Description
Mouse monoclonal [464.6] to alpha 1 Sodium Potassium ATPase -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Human, Pig
Predicted to work with: Sheep, Dog, Xenopus laevis, Monkey
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Immunogen
Full length native protein (purified) corresponding to Rabbit alpha 1 Sodium Potassium ATPase. Rabbit renal outermedulla.
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Positive control
- This antibody gave a positive signal in the following tissue lysates: human kidney, rabbit heart, human brain and human brain membrane.This antibody also gave a positive signal in Porcine proximal tubule lysate.
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General notes
This antibody clone [464.6] is manufactured by Abcam.
If you require this antibody in a different buffer formulation or a different conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Some batches contain 6.97% L-Arginine as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team. -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
464.6 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 647 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab196695)
- HRP Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab196696)
- Alexa Fluor® 488 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab197496)
- Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (ab237969)
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (4) |
Use a concentration of 5 µg/ml.
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| WB | (22) |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 112 kDa. Abcam recommends using 5% BSA as the blocking agent.
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| ICC |
Use a concentration of 1 - 10 µg/ml.
We recommend Methanol fixation and Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (ab150118) secondary antibody. |
| Notes |
|---|
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IHC-P
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 112 kDa. Abcam recommends using 5% BSA as the blocking agent. |
|
ICC
Use a concentration of 1 - 10 µg/ml. We recommend Methanol fixation and Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (ab150118) secondary antibody. |
Target
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Function
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. -
Sequence similarities
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. -
Post-translational
modificationsPhosphorylation on Tyr-10 modulates pumping activity. -
Cellular localization
Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 403992 Dog
- Entrez Gene: 476 Human
- Entrez Gene: 11928 Mouse
- Entrez Gene: 397481 Pig
- Entrez Gene: 24211 Rat
- Entrez Gene: 443381 Sheep
- Entrez Gene: 399285 Xenopus laevis
- Omim: 182310 Human
see all -
Alternative names
- A1A1 antibody
- AT1A1 antibody
- AT1A1_HUMAN antibody
see all
Images
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Immunocytochemistry - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)
ab7671 staining alpha 1 Sodium Potassium ATPase in Hek293 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab7671 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a single confocal section is shown.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)](https://www.abcam.com/ps/products/7/ab7671/Images/ab7671-240726-anti-alpha-1-sodium-potassium-atpase-antibody-4646-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)This image is courtesy of an Abreview submitted by Anne Sailerab7671 staining alpha 1 Sodium Potassium ATPase in human formaldehyde tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 12 hours at 4°C; antigen retrieval was by heat mediation in a buffer pH 9. Samples were incubated with primary antibody (5µg/ml in 5% BSA) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
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![Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)](https://www.abcam.com/ps/products/7/ab7671/Images/alpha-1-Sodium-Potassium-ATPase-Primary-antibodies-ab7671-65.jpg)
Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)Lanes 1-3 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 1 µg/ml
Lanes 4-6 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 2 µg/ml
Lanes 7-9 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 5 µg/ml
Lanes 1 & 4 & 7 : Human brain tissue lysate - total protein (ab29466)
Lanes 2 & 5 & 8 : Brain (Mouse) Tissue Lysate (ab27253)
Lanes 3 & 6 & 9 : Brain (Rat) Tissue Lysate (ab7942)
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 112 kDa
Exposure time: 3 minutes -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)](https://www.abcam.com/ps/products/7/ab7671/Images/ab7671-13008-anti-alpha-1-sodium-potassium-atpase-antibody-4646-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)IHC image of Ab7671 staining in Human Kidney Carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Ab7671, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)](https://www.abcam.com/ps/products/7/ab7671/Images/alpha-1-Sodium-Potassium-ATPase-Primary-antibodies-ab7671-9.jpg)
Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)All lanes : alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker at 10 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : Human kidney tissue lysate - total protein (ab30203)
Lane 3 : Heart (Rabbit) Whole Cell Lysate - normal tissue (ab29072)
Lane 4 : Human brain tissue lysate - total protein (ab29466)
Lane 5 : Brain (Human) Membrane Lysate - adult normal tissue
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 112 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
![Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)](https://www.abcam.com/ps/products/7/ab7671/Images/ab7671_3.jpg)
Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 1/5000 dilution + Porcine proximal tubule lysate
Predicted band size: 112 kDa
Western blot analysis detecting Na, K-ATP-ase (alpha) in porcine proximal tubule protein, using Ab7671. Band is at 112 kDa.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (245)
ab7671 has been referenced in 245 publications.
- Sethuraman SN et al. Novel calreticulin-nanoparticle in combination with focused ultrasound induces immunogenic cell death in melanoma to enhance antitumor immunity. Theranostics 10:3397-3412 (2020). PubMed: 32206098
- Legrand C et al. The Na,K-ATPase acts upstream of phosphoinositide PI(4,5)P2 facilitating unconventional secretion of Fibroblast Growth Factor 2. Commun Biol 3:141 (2020). PubMed: 32214225
- Han J et al. Maresin Conjugates in Tissue Regeneration 1 improves alveolar fluid clearance by up-regulating alveolar ENaC, Na, K-ATPase in lipopolysaccharide-induced acute lung injury. J Cell Mol Med 24:4736-4747 (2020). PubMed: 32160403
- Vangeel L et al. The Zinc-Finger Domain Containing Protein ZC4H2 Interacts with TRPV4, Enhancing Channel Activity and Turnover at the Plasma Membrane. Int J Mol Sci 21:N/A (2020). PubMed: 32443528
- Yang CW et al. Natural cardenolides suppress coronaviral replication by downregulating JAK1 via a Na+/K+-ATPase independent proteolysis. Biochem Pharmacol 180:114122 (2020). PubMed: 32592721