Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)
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Overview
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Product nameAnti-alpha 1 Sodium Potassium ATPase antibody [464.6]
See all alpha 1 Sodium Potassium ATPase primary antibodies -
DescriptionMouse monoclonal [464.6] to alpha 1 Sodium Potassium ATPase
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Host speciesMouse
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SpecificityThis antibody is specific for Na,K-ATPase alpha 1 subunit.
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Tested applicationsSuitable for: IHC-P, ICC/IF, WB, Flow Cytmore details
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Species reactivityReacts with: Mouse, Rat, Sheep, Rabbit, Dog, Human, Pig, Xenopus laevis, Monkey
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Immunogen
Full length native protein (purified) corresponding to Rabbit alpha 1 Sodium Potassium ATPase. Rabbit renal outermedulla.
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Positive control
- This antibody gave a positive signal in HEK293 whole cell lysate and in the following tissue lysates: human kidney, rabbit heart, human brain and human brain membrane.This antibody also gave a positive signal in Porcine proximal tubule lysate.
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General notes
This antibody clone [464.6] is manufactured by Abcam.
If you require this antibody in a different buffer formulation or a different conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Abcam recommended secondaries - Goat Anti-Mouse HRP (ab205719) and Goat Anti-Mouse Alexa Fluor® 488 (ab150113).
See other anti-mouse secondary antibodies that can be used with this antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Some batches contain 6.97% L-Arginine as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team. -
Concentration information loading... -
PurityIgG fraction
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ClonalityMonoclonal
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Clone number464.6
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IsotypeIgG1
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Light chain typekappa
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab7671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. | |
| ICC/IF | Use a concentration of 5 - 10 µg/ml. We recommend Methanol fixation and Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (ab150118) secondary antibody. |
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| WB | Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 112 kDa. Abcam recommends using 5% BSA as the blocking agent. | |
| Flow Cyt | Use a concentration of 10 µg/ml. See Abreview. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. We recommend Donkey Anti-Mouse IgG H&L (Phycoerythrin) preadsorbed (ab7003) secondary antibody. |
Target
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FunctionThis is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
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Sequence similaritiesBelongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
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Post-translational
modificationsPhosphorylation on Tyr-10 modulates pumping activity. -
Cellular localizationCell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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Database links
- Entrez Gene: 403992 Dog
- Entrez Gene: 476 Human
- Entrez Gene: 11928 Mouse
- Entrez Gene: 397481 Pig
- Entrez Gene: 24211 Rat
- Entrez Gene: 443381 Sheep
- Entrez Gene: 399285 Xenopus laevis
- Omim: 182310 Human
see all -
Alternative names
- A1A1 antibody
- AT1A1 antibody
- AT1A1_HUMAN antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)](https://www.abcam.com/ps/products/7/ab7671/Images/ab7671-240726-ab7671ihcp.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)This image is courtesy of an Abreview submitted by Anne Sailerab7671 staining alpha 1 Sodium Potassium ATPase in human formaldehyde tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 12 hours at 4°C; antigen retrieval was by heat mediation in a buffer pH 9. Samples were incubated with primary antibody (5µg/ml in 5% BSA) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
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![Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)](https://www.abcam.com/ps/products/7/ab7671/Images/alpha-1-Sodium-Potassium-ATPase-Primary-antibodies-ab7671-65.jpg)
Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)Lanes 1-3 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 1 µg/ml
Lanes 4-6 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 2 µg/ml
Lanes 7-9 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 5 µg/ml
Lanes 1 & 4 & 7 : Human brain tissue lysate - total protein (ab29466)
Lanes 2 & 5 & 8 : Brain (Mouse) Tissue Lysate (ab27253)
Lanes 3 & 6 & 9 : Brain (Rat) Tissue Lysate (ab7942)
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 112 kDa
Exposure time: 3 minutes -
![Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)](https://www.abcam.com/ps/products/7/ab7671/Images/alpha-1-Sodium-Potassium-ATPase-Primary-antibodies-ab7671-15.jpeg)
Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)ICC/IF image of ab7671 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7671, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, HepG2 and MCF7 cells at 5µg/ml. -
![Flow Cytometry - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)](https://www.abcam.com/ps/products/7/ab7671/Images/Sodium-Potassium-ATPase-Primary-antibodies-ab7671-3.jpg)
Flow Cytometry - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)This image is courtesy of an anonymous Abreviewab7671 staining Sodium Potassium ATPase - Plasma Membrane Marker in Human Fibrosarcoma HT1080 cells by Flow Cytometry. Cells were fixed with paraformaldehyde. The sample was incubated with the primary antibody 10 µg/ml in PBS for 1 hour. An Abcam Donkey Anti-Mouse IgG H&L (Phycoerythrin) preadsorbed (ab7003) secondary antibody, 5 µg/ml, was used.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)](https://www.abcam.com/ps/products/7/ab7671/Images/alpha-1-Sodium-Potassium-ATPase-Primary-antibodies-ab7671-13.jpeg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)IHC image of Ab7671 staining in Human Kidney Carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Ab7671, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)](https://www.abcam.com/ps/products/7/ab7671/Images/alpha-1-Sodium-Potassium-ATPase-Primary-antibodies-ab7671-9.jpg)
Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)All lanes : alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker at 10 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : Human kidney tissue lysate - total protein (ab30203)
Lane 3 : Heart (Rabbit) Whole Cell Lysate - normal tissue (ab29072)
Lane 4 : Human brain tissue lysate - total protein (ab29466)
Lane 5 : Brain (Human) Membrane Lysate - adult normal tissue
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 112 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
![Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)](https://www.abcam.com/ps/products/7/ab7671/Images/ab7671_2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)This image is courtesy of an anonymous Abreviewab7671 staining (dog) MDCK II cells by ICC/IF. Cells were acetone fixed and blocked with 4% serum for 10 minutes at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 16 hours at 4°C. Ab6785 (a FITC conjugated goat polyclonal to mouse IgG - H&L) was diluted 1/100 and employed as the secondary antibody.
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![Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)](https://www.abcam.com/ps/products/7/ab7671/Images/ab7671_3.jpg)
Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 1/5000 dilution + Porcine proximal tubule lysate
Predicted band size: 112 kDa
Western blot analysis detecting Na, K-ATP-ase (alpha) in porcine proximal tubule protein, using Ab7671. Band is at 112 kDa. -
![Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)](https://www.abcam.com/ps/products/7/ab7671/Images/alpha-1-Sodium-Potassium-ATPase-Primary-antibodies-ab7671-46.jpg)
Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)All lanes : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 1/1000 dilution
All lanes : Parotid acinar cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated Donkey anti-Mouse polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 112 kDa
Observed band size: 112 kDa
Exposure time: 5 minutes
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![Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)](https://www.abcam.com/ps/products/7/ab7671/Images/ab7671-273815-ab767158321.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)This image is courtesy of an anonymous abreview.Immunocytochemistry/ Immunofluorescence analysis of B16 mice tumor cells labeling alpha 1 Sodium Potassium ATPase with ab7671 at 1/200 dilution. Cells were fixed in formaldehyde and permeabilized with 0.25% Triton-X100 for 10 minutes. Cells were blocked with 1% BSA for 1 hour at 20°C, followed by staining with ab7671 at 1/200 for 18 hours at 4°C in 1% BSA in PBST buffer. Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (ab150118) secondary antibody was used at 1/1000 dilution. DAPI was used to counterstain.
References
This product has been referenced in:
- Xie JC et al. Hypoxia increases amyloid-ß level in exosomes by enhancing the interaction between CD147 and Hook1. Am J Transl Res 10:150-163 (2018). Read more (PubMed: 29423001) »
- Kasendra M et al. Development of a primary human Small Intestine-on-a-Chip using biopsy-derived organoids. Sci Rep 8:2871 (2018). Read more (PubMed: 29440725) »
