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Full length native protein (purified) corresponding to Rabbit alpha 1 Sodium Potassium ATPase. Rabbit renal outermedulla.
Our Abpromise guarantee covers the use of ab7671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|ICC/IF||Use a concentration of 5 - 10 µg/ml.
We recommend Methanol fixation and Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (ab150118) secondary antibody.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 112 kDa. Abcam recommends using 5% BSA as the blocking agent.|
|Flow Cyt||Use a concentration of 10 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ab7671 staining alpha 1 Sodium Potassium ATPase in human formaldehyde tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 12 hours at 4°C; antigen retrieval was by heat mediation in a buffer pH 9. Samples were incubated with primary antibody (5µg/ml in 5% BSA) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
ab7671 staining Sodium Potassium ATPase - Plasma Membrane Marker in Human Fibrosarcoma HT1080 cells by Flow Cytometry. Cells were fixed with paraformaldehyde. The sample was incubated with the primary antibody 10 µg/ml in PBS for 1 hour. An Abcam Donkey Anti-Mouse IgG H&L (Phycoerythrin) preadsorbed (ab7003) secondary antibody, 5 µg/ml, was used.
ab7671 staining (dog) MDCK II cells by ICC/IF. Cells were acetone fixed and blocked with 4% serum for 10 minutes at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 16 hours at 4°C. Ab6785 (a FITC conjugated goat polyclonal to mouse IgG - H&L) was diluted 1/100 and employed as the secondary antibody.
Immunocytochemistry/ Immunofluorescence analysis of B16 mice tumor cells labeling alpha 1 Sodium Potassium ATPase with ab7671 at 1/200 dilution. Cells were fixed in formaldehyde and permeabilized with 0.25% Triton-X100 for 10 minutes. Cells were blocked with 1% BSA for 1 hour at 20°C, followed by staining with ab7671 at 1/200 for 18 hours at 4°C in 1% BSA in PBST buffer. Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (ab150118) secondary antibody was used at 1/1000 dilution. DAPI was used to counterstain.
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