Key features and details
- Mouse monoclonal [464.6] to alpha 1 Sodium Potassium ATPase (Alexa Fluor® 647)
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Isotype: IgG1
Product nameAnti-alpha 1 Sodium Potassium ATPase antibody [464.6] (Alexa Fluor® 647)
See all alpha 1 Sodium Potassium ATPase primary antibodies
DescriptionMouse monoclonal [464.6] to alpha 1 Sodium Potassium ATPase (Alexa Fluor® 647)
ConjugationAlexa Fluor® 647. Ex: 652nm, Em: 668nm
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Human
Full length native protein (purified) corresponding to Rabbit alpha 1 Sodium Potassium ATPase.
- ICC/IF: HEK293 cells.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or firstname.lastname@example.org.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Light chain typekappa
- Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (HRP) (ab196696)
- Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (Alexa Fluor® 488) (ab197496)
- Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (ab237969)
- Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)
Our Abpromise guarantee covers the use of ab196695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product gave a positive signal in Hek293 cells fixed with 100% methanol (5 min)
FunctionThis is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Sequence similaritiesBelongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
modificationsPhosphorylation on Tyr-10 modulates pumping activity.
Cellular localizationCell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- A1A1 antibody
- AT1A1 antibody
- AT1A1_HUMAN antibody
ab196695 staining alpha 1 Sodium Potassium ATPase in Hek293 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196695 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab196695 has not yet been referenced specifically in any publications.