Key features and details
- Mouse monoclonal [464.6] to alpha 1 Sodium Potassium ATPase (HRP)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
- Isotype: IgG1
Product nameAnti-alpha 1 Sodium Potassium ATPase antibody [464.6] (HRP)
See all alpha 1 Sodium Potassium ATPase primary antibodies
DescriptionMouse monoclonal [464.6] to alpha 1 Sodium Potassium ATPase (HRP)
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Full length native protein (purified) corresponding to Rabbit alpha 1 Sodium Potassium ATPase.
- WB: Human Brain, Mouse Brain and Rat Brain tissue lysates. IHC-P: Normal human kidney tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
Light chain typekappa
- Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (Alexa Fluor® 647) (ab196695)
- Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (Alexa Fluor® 488) (ab197496)
- Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (ab237969)
- Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)
Our Abpromise guarantee covers the use of ab196696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 98 kDa (predicted molecular weight: 112 kDa).
Abcam recommends using 5% BSA as the blocking agent.
|IHC-P||1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionThis is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Sequence similaritiesBelongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
modificationsPhosphorylation on Tyr-10 modulates pumping activity.
Cellular localizationCell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- A1A1 antibody
- AT1A1 antibody
- AT1A1_HUMAN antibody
IHC image of alpha 1 Sodium Potassium ATPase staining in a section of formalin-fixed paraffin-embedded normal human kidney*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab196696 at 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
All lanes : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (HRP) (ab196696) at 1/5000 dilution
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 112 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted?
Exposure time: 2 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab196696 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab196696 has not yet been referenced specifically in any publications.