Product nameAnti-alpha 1 Sodium Potassium ATPase antibody
See all alpha 1 Sodium Potassium ATPase primary antibodies
DescriptionRabbit polyclonal to alpha 1 Sodium Potassium ATPase
Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide from the N terminal region of Human alpha 1 Sodium Potassium ATPase.
- Human pancreas tissue, 293 cells treated with 125 ng/ml of PMA for 30 minutes, and COS7 cells.
Storage instructionsShipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.88% Sodium chloride, 49% PBS
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab133211 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 113 kDa.|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/500.|
FunctionThis is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Sequence similaritiesBelongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
modificationsPhosphorylation on Tyr-10 modulates pumping activity.
Cellular localizationCell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- A1A1 antibody
- AT1A1 antibody
- AT1A1_HUMAN antibody
All lanes : Anti-alpha 1 Sodium Potassium ATPase antibody (ab133211) at 1/500 dilution
Lane 1 : Extract from 293 cells treated with 125 ng/ml of PMA for 30 minutes
Lane 2 : Extract from 293 cells treated with 125 ng/ml of PMA for 30 minutes with immunizing peptide
Predicted band size: 113 kDa
Immunofluorescent analysis of COS7 cells labelling alpha 1 Sodium Potassium ATPase with ab133211 at 1/100 dilution (left). Right panel is treated with the immunizing peptide.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas labelling alpha 1 Sodium Potassium ATPase with ab133211 at 1/50, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
ab133211 has not yet been referenced specifically in any publications.