Key features and details
- Rabbit polyclonal to alpha 1 Sodium Potassium ATPase
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-alpha 1 Sodium Potassium ATPase antibody
See all alpha 1 Sodium Potassium ATPase primary antibodies
DescriptionRabbit polyclonal to alpha 1 Sodium Potassium ATPase
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Cow, Dog, Pig
Synthetic peptide corresponding to Human alpha 1 Sodium Potassium ATPase (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: P05023
- WB: HEK-293T and HeLa whole cell lysate. IHC-P: Human kidney tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230541 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 113 kDa.|
FunctionThis is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Sequence similaritiesBelongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
modificationsPhosphorylation on Tyr-10 modulates pumping activity.
Cellular localizationCell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- A1A1 antibody
- AT1A1 antibody
- AT1A1_HUMAN antibody
Formalin-fixed, paraffin-embedded human kidney tissue stained for alpha 1 Sodium Potassium ATPase using ab230541 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-alpha 1 Sodium Potassium ATPase antibody (ab230541) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Predicted band size: 113 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230541 has not yet been referenced specifically in any publications.