Key features and details
- Rabbit polyclonal to alpha 1 Sodium Potassium ATPase (phospho S16)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-alpha 1 Sodium Potassium ATPase (phospho S16) antibody
See all alpha 1 Sodium Potassium ATPase primary antibodies
DescriptionRabbit polyclonal to alpha 1 Sodium Potassium ATPase (phospho S16)
Specificityab194532 detects endogenous level of alpha 1 Sodium Potassium ATPase only when phosphorylated at serine 16.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human alpha 1 Sodium Potassium ATPase (phospho S16) conjugated to Keyhole Limpet Haemocyanin (KLH). Peptide sequence around phosphorylation site of serine 16 (A-V-S(p)-E-Q) derived from Human alpha 1 Sodium Potassium ATPase.
Database link: P05023
- 293 cell extract, treated with PMA.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 49% PBS
PBS (without Mg2+and Ca2+)
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab194532 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab194532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 113 kDa.|
FunctionThis is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Sequence similaritiesBelongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
modificationsPhosphorylation on Tyr-10 modulates pumping activity.
Cellular localizationCell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- A1A1 antibody
- AT1A1 antibody
- AT1A1_HUMAN antibody
ab194532 has not yet been referenced specifically in any publications.