Recombinant
RabMAb

Recombinant Anti-alpha 1 Sodium Potassium ATPase (phospho Y10) antibody [EPR2375(2)] (ab124677)

Rabbit recombinant monoclonal alpha 1 Sodium Potassium ATPase (phospho Y10) antibody [EPR2375(2)]. Validated in WB, IP and tested in Mouse, Rat, Human.

Overview

  • Product name

    Anti-alpha 1 Sodium Potassium ATPase (phospho Y10) antibody [EPR2375(2)]
    See all alpha 1 Sodium Potassium ATPase primary antibodies
  • Description

    Rabbit monoclonal [EPR2375(2)] to alpha 1 Sodium Potassium ATPase (phospho Y10)
  • Host species

    Rabbit
  • Specificity

    ab124677 only detects alpha 1 Sodium Potassium ATPase phosphorylated at Tyrosine 10.
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding Tyrosine 10 of Human alpha 1 Sodium Potassium ATPase.

  • Positive control

    • HeLa cell lysate, treated with Pervanadate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR2375(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 100 kDa (predicted molecular weight: 113 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function

      This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
    • Sequence similarities

      Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
    • Post-translational
      modifications

      Phosphorylation on Tyr-10 modulates pumping activity.
    • Cellular localization

      Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links

    • Alternative names

      • A1A1 antibody
      • AT1A1 antibody
      • AT1A1_HUMAN antibody
      • ATP1A1 antibody
      • Atpa-1 antibody
      • ATPase Na+/K+ transporting alpha 1 polypeptide antibody
      • ATPase Na+/K+ transporting subunit alpha 1 antibody
      • BC010319 antibody
      • EC 3.6.3.9 antibody
      • MGC3285 antibody
      • MGC38419 antibody
      • MGC51750 antibody
      • Na K ATPase alpha A catalytic polypeptide antibody
      • Na K ATPase catalytic subunit alpha A protein antibody
      • Na(+)/K(+) ATPase 1 antibody
      • Na(+)/K(+) ATPase alpha-1 subunit antibody
      • Na+, K+ ATPase alpha subunit antibody
      • Na+/K+ ATPase alpha 1 subunit antibody
      • Na+/K+ ATPase 1 antibody
      • Na,K ATPase alpha 1 subunit antibody
      • Nkaa1b antibody
      • Sodium potassium ATPase alpha 1 polypeptide antibody
      • Sodium pump 1 antibody
      • Sodium pump subunit alpha-1 antibody
      • sodium-potassium ATPase catalytic subunit alpha-1 antibody
      • Sodium/potassium-transporting ATPase subunit alpha-1 antibody
      see all

    Images

    • All lanes : Anti-alpha 1 Sodium Potassium ATPase (phospho Y10) antibody [EPR2375(2)] (ab124677) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HeLa cell lysate, treated with Pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 113 kDa
      Observed band size: 100 kDa
      why is the actual band size different from the predicted?

    References

    ab124677 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab124677.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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