Product nameAnti-alpha 1 Sodium Potassium ATPase (phospho Y10) antibody [EPR2375(2)]
See all alpha 1 Sodium Potassium ATPase primary antibodies
DescriptionRabbit monoclonal [EPR2375(2)] to alpha 1 Sodium Potassium ATPase (phospho Y10)
Specificityab124677 only detects alpha 1 Sodium Potassium ATPase phosphorylated at Tyrosine 10.
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
A phospho specific peptide corresponding to residues surrounding Tyrosine 10 of Human alpha 1 Sodium Potassium ATPase.
- HeLa cell lysate, treated with Pervanadate.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124677 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 100 kDa (predicted molecular weight: 113 kDa).|
|IP||1/10 - 1/100.|
FunctionThis is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Sequence similaritiesBelongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
modificationsPhosphorylation on Tyr-10 modulates pumping activity.
Cellular localizationCell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- A1A1 antibody
- AT1A1 antibody
- AT1A1_HUMAN antibody
All lanes : Anti-alpha 1 Sodium Potassium ATPase (phospho Y10) antibody [EPR2375(2)] (ab124677) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate, treated with Pervanadate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 113 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
ab124677 has not yet been referenced specifically in any publications.