• Product name
    Anti-alpha 1 Spectrin antibody [17C7]
    See all alpha 1 Spectrin primary antibodies
  • Description
    Mouse monoclonal [17C7] to alpha 1 Spectrin
  • Host species
  • Specificity
    ab11751 is developed against the alpha I spectrin SH3 domain. It does not crossreact with alpha II (nonerythroid) spectrin SH3 domain or recombinant human fodrin SH3 domain. In NIH 3T3 cells, ab11751 reacts with a 200 kDa protein which is a candidate marker of endocytic vesicles called macropinosomes.
  • Tested applications
    Suitable for: ICC/IF, ELISA, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant alpha I spectrin (erythroid spectrin) SH3 domain.

  • Positive control
    • Erythroid spectrin in red blood cells and cerebellum.



Our Abpromise guarantee covers the use of ab11751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100. (See Abreview)
ELISA 1/100000 - 1/1e+006.
WB 1/100 - 1/2000. Predicted molecular weight: 280 kDa. PubMed: 23780662
IHC-Fr 1/10 - 1/50.


  • Function
    Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.
  • Involvement in disease
    Defects in SPTA1 are the cause of elliptocytosis type 2 (EL2) [MIM:130600]. EL2 is a Rhesus-unlinked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape.
    Defects in SPTA1 are a cause of hereditary pyropoikilocytosis (HPP) [MIM:266140]. HPP is an autosomal recessive disorder characterized by hemolytic anemia, microspherocytosis, poikilocytosis, and an unusual thermal sensitivity of red cells.
    Defects in SPTA1 are the cause of spherocytosis type 3 (SPH3) [MIM:270970]; also known as hereditary spherocytosis type 3 (HS3). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. SPH3 is characterized by severe hemolytic anemia. Inheritance is autosomal recessive.
  • Sequence similarities
    Belongs to the spectrin family.
    Contains 3 EF-hand domains.
    Contains 1 SH3 domain.
    Contains 21 spectrin repeats.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cytoplasm > cell cortex.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha I spectrin antibody
    • EL 2 antibody
    • EL2 antibody
    • Elliptocytosis 2 antibody
    • Elliptocytosis2 antibody
    • Erythrocyte alpha spectrin antibody
    • erythrocyte antibody
    • Erythroid alpha spectrin antibody
    • Erythroid alpha-spectrin antibody
    • Erythroid spectrin alpha antibody
    • HPP antibody
    • HS3 antibody
    • Spectrin alpha chain antibody
    • Spectrin alpha chain erythrocyte antibody
    • Spectrin alpha erythrocytic 1 antibody
    • SPH3 antibody
    • SPTA 1 antibody
    • SPTA antibody
    • SPTA1 antibody
    • SPTA1_HUMAN antibody
    see all


  • ab11751 staining alpha 1 Spectrin in rat artery smooth muscle cells by Immunocytochemistry/ Immunofluorescence
    Cells were fixed with paraformaldehyde, permeabilized using Triton X-100 and then incubated with ab11751 used at a 1/100 dilution for 1 hour at 21°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse monoclonal used at a 1/100 dilution.

    Rat artery smooth muscle cells were triple stained for spectrin (green), Actin (red) and DNA blue. The cell in the image is in telophase and spectrin is localized at the midbody.

    See Abreview

  • Central Panel: ab11751 staining alpha 1 Spectrin in human PMN cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde and blocked with 2% BSA for 1 hour at 22°C. Samples were incubated with primary antibody 1/100 in blocking buffer for 4 hours at 37°C. An Alexa Fluor® 488-conjugated Goat polyclonal to mouse IgG, dilution 1/250, was used as secondary antibody.
    Left-hand panel: Nuclei counterstained with DAPI (blue).
    Right-hand panel: Overlay

    See Abreview

  • Anti-alpha 1 Spectrin antibody [17C7] (ab11751) at 1/1000 dilution + Human Platelet whole cell lysate at 25 µg

    HRP-conjugated Goat Anti-mouse IgG at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 280 kDa
    Additional bands at: 200 kDa (possible cleavage fragment)

    Exposure time: 1 hour

    See Abreview


This product has been referenced in:
  • Wang Y  et al. Critical roles of aII spectrin in brain development and epileptic encephalopathy. J Clin Invest 128:760-773 (2018). Read more (PubMed: 29337302) »
  • Huang HM  et al. Ankyrin-1 Gene Exhibits Allelic Heterogeneity in Conferring Protection Against Malaria. G3 (Bethesda) 7:3133-3144 (2017). WB ; Mouse . Read more (PubMed: 28751503) »
See all 9 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A


Thank you for your enquiry.

I am sorry to confirm that as far as we are aware, ab11751 has never been tested in immunohisochemistry on paraffin embedded sections (IHC-P). All tested and guaranteed applications and speciesare specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

If you would like to test the antibody in this untested application, please contact me agian prior to the purchase by replying to this message as you may be eligible for our testing discount program.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:


I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Rat Cell (rat artery smooth muscle cells)
rat artery smooth muscle cells
Yes - Triton x100

Dr. Rosalind Silverman-Gavrila

Verified customer

Submitted Jan 20 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (Platelet Lysate (precleared))
Loading amount
25 µg
Platelet Lysate (precleared)
Gel Running Conditions
Reduced Denaturing (4-15)
Blocking step
Thermo Starting Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C

Dr. Mahesh Shivananjappa

Verified customer

Submitted Jan 08 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Human Cell (PMN)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C

Dr. Mahesh Shivananjappa

Verified customer

Submitted Dec 22 2009


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