Key features and details
- Mouse monoclonal [AF10] to alpha 1 Spectrin
- Suitable for: WB, IP, ICC
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-alpha 1 Spectrin antibody [AF10]
See all alpha 1 Spectrin primary antibodies
DescriptionMouse monoclonal [AF10] to alpha 1 Spectrin
Specificityab86184 is specific to the erythroid alpha 1 Spectrin.
Tested applicationsSuitable for: WB, IP, ICCmore details
Species reactivityReacts with: Human
Ghost proteins of human red blood cells.
ab86184 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein G purified
Primary antibody notesab86184 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.
Our Abpromise guarantee covers the use of ab86184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 280 kDa.|
|IP||Use at an assay dependent dilution.|
|ICC||Use at an assay dependent dilution.|
FunctionSpectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.
Involvement in diseaseDefects in SPTA1 are the cause of elliptocytosis type 2 (EL2) [MIM:130600]. EL2 is a Rhesus-unlinked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape.
Defects in SPTA1 are a cause of hereditary pyropoikilocytosis (HPP) [MIM:266140]. HPP is an autosomal recessive disorder characterized by hemolytic anemia, microspherocytosis, poikilocytosis, and an unusual thermal sensitivity of red cells.
Defects in SPTA1 are the cause of spherocytosis type 3 (SPH3) [MIM:270970]; also known as hereditary spherocytosis type 3 (HS3). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. SPH3 is characterized by severe hemolytic anemia. Inheritance is autosomal recessive.
Sequence similaritiesBelongs to the spectrin family.
Contains 3 EF-hand domains.
Contains 1 SH3 domain.
Contains 21 spectrin repeats.
Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cell cortex.
- Information by UniProt
- Alpha I spectrin antibody
- EL 2 antibody
- EL2 antibody
ab86184 has not yet been referenced specifically in any publications.